Label-free liquid crystal-based biosensor for detection of dopamine using DNA aptamer as a recognition probe
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nguyen, D.K. | - |
dc.contributor.author | Jang, C.-H. | - |
dc.date.available | 2020-10-20T01:02:53Z | - |
dc.date.created | 2020-08-10 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78411 | - |
dc.description.abstract | We present a label-free liquid crystal-based biosensor for the detection of dopamine (DA) in aqueous solutions using dopamine-binding aptamers (DBA) as recognition elements. In this system, the dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) self-assembled monolayers immobilized on glass slides support the long alkyl chains that keep the liquid crystal (LC) molecules in a homeotropic orientation. Glutaraldehyde (GA) is used as a cross-linker to immobilize DBA onto the surface of glass slides. The specific binding of DA and DBA disrupts the homeotropic orientation of LCs, thereby inducing a change in the orientation from homeotropic to a random alignment. This orientation change can be converted and visualized simply as a transition from a dark optical LC image to a brighter image under a polarized optical microscope (POM), enabling the detection of DA. The developed LC-based aptasensor shows a good linear optical response towards DA in the very wide range of 1 pM–10 μM (0.19 pg/mL to 1.9 μg/mL) and has a very low detection limit of 10 pM (∼1.9 pg/mL). The biosensor also exhibited satisfactory selectivity and could be successfully applied to detect DA in human urine. The proposed LC-based aptamer sensing method offers a simple, rapid, highly sensitive and selective, and a label-free method for the analysis of DA in real clinical samples. © 2020 Elsevier Inc. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Academic Press Inc. | - |
dc.relation.isPartOf | Analytical Biochemistry | - |
dc.title | Label-free liquid crystal-based biosensor for detection of dopamine using DNA aptamer as a recognition probe | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000569074500001 | - |
dc.identifier.doi | 10.1016/j.ab.2020.113807 | - |
dc.identifier.bibliographicCitation | Analytical Biochemistry, v.605 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85088855869 | - |
dc.citation.title | Analytical Biochemistry | - |
dc.citation.volume | 605 | - |
dc.contributor.affiliatedAuthor | Nguyen, D.K. | - |
dc.contributor.affiliatedAuthor | Jang, C.-H. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Dopamine | - |
dc.subject.keywordAuthor | Dopamine-binding aptamer | - |
dc.subject.keywordAuthor | LC-Based aptasensors | - |
dc.subject.keywordAuthor | Liquid crystals | - |
dc.subject.keywordPlus | Amines | - |
dc.subject.keywordPlus | Biosensors | - |
dc.subject.keywordPlus | Chlorine compounds | - |
dc.subject.keywordPlus | Glass | - |
dc.subject.keywordPlus | Liquid crystals | - |
dc.subject.keywordPlus | Neurophysiology | - |
dc.subject.keywordPlus | Ammonium chloride | - |
dc.subject.keywordPlus | Homeotropic orientation | - |
dc.subject.keywordPlus | Linear optical response | - |
dc.subject.keywordPlus | Long alkyl chains | - |
dc.subject.keywordPlus | Low detection limit | - |
dc.subject.keywordPlus | Orientation changes | - |
dc.subject.keywordPlus | Polarized optical microscopes | - |
dc.subject.keywordPlus | Recognition element | - |
dc.subject.keywordPlus | Crystal orientation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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