Flavimarina flava sp nov., isolated from Salicornia herbacea

Abstract

A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091(T). It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73(T) with 94.8% similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F. pacifica KCTC 32466(T) and Leeuwenhoekiella marinoflava DSM 3653(T) were 80.5 and 80.2 %, respectively. Growth of strain MBLN091(T) was observed in the presence of 0.5-15.0% (w/v) NaCl at 4-35 degrees C and pH 6.0-8.0, with optimal growth in the presence of 2.5-5.0% (w/v) NaCl at 20-25 degrees C and pH 7.0. This isolate was able to hydrolyse gelatin. The only respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. Major fatty acids of the isolate were iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-17:0 3-OH and iso-C-15:1 G. The genomic DNA G+C content was 39.6 mol%. The physiological features were closely related to F. pacifica. Therefore, strain MBLN091(T) is considered to represent a novel species within the genus Flavimarina, for which the name Flavimarina flava sp. nov. is proposed. The type strain is MBLN091(T) (=KCTC 52527(T)=JCM 31731(T)).