Validation of an HPLC/UV Analysis Method for Cirsimaritin in Cirsium japonicum var. maackii

Abstract

Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hepatitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r2 ³ 0.9999), accuracy (93.9-111.3%), and precision (RSD £ 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM.