Nectin-2 (CD112) Is Expressed on Outgrowth Endothelial Cells and Regulates Cell Proliferation and Angiogenic Function
DC Field | Value | Language |
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dc.contributor.author | Son, YeonSung | - |
dc.contributor.author | Lee, BomNaeRin | - |
dc.contributor.author | Choi, Young-Jin | - |
dc.contributor.author | Jeon, Seon Ae | - |
dc.contributor.author | Kim, Ju-Hyun | - |
dc.contributor.author | Lee, Hoo-Keun | - |
dc.contributor.author | Kwon, Sang-Mo | - |
dc.contributor.author | Cho, Je-Yoel | - |
dc.date.available | 2020-02-28T00:43:34Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-09-27 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7873 | - |
dc.description.abstract | Outgrowth endothelial cells (OECs) are a subpopulation of endothelial progenitor cells (EPCs) that have the capacity for proliferation and the ability to promote angiogenesis. In this study, we identified Nectin-2 as a surface protein of OECs through unbiased quantitative proteomics analysis. Using immunocytochemistry and flow cytometry, we confirmed that Nectin-2 is highly expressed on OECs. Nectin-2 (CD112) expression was limited or lower on mononuclear cells (MNCs) and mature tube-forming endothelial cells (ECs). Blocking Nectin-2 with a neutralizing monoclonal antibody significantly increased the transwell migration and tube forming capacity of OECs. Similarly, Nectin-2 knockdown resulted in enhanced tube formation, cell migration and proliferation with p-Erk activation. Moreover, Nectin-2 deficiency resulted in compensatory increase of other Nectin family genes including Nectin-3 and Necl-4 which promote VEGFR signaling. These results indicate that Nectin-2 is a surface marker and an important regulator of OECs, with significant implications for the isolation of OECs and blocking Nectin-2 on OECs by an antibody for angiogenic applications. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.subject | PROGENITOR CELLS | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | PVR CD155 | - |
dc.subject | ADHESION | - |
dc.subject | AFADIN | - |
dc.subject | INHIBITION | - |
dc.subject | MOLECULES | - |
dc.subject | MOVEMENT | - |
dc.subject | CADHERIN | - |
dc.subject | VEGFR-2 | - |
dc.title | Nectin-2 (CD112) Is Expressed on Outgrowth Endothelial Cells and Regulates Cell Proliferation and Angiogenic Function | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000384169900015 | - |
dc.identifier.doi | 10.1371/journal.pone.0163301 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.11, no.9 | - |
dc.identifier.scopusid | 2-s2.0-84991585584 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 11 | - |
dc.citation.number | 9 | - |
dc.contributor.affiliatedAuthor | Lee, Hoo-Keun | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | PVR CD155 | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | AFADIN | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | MOVEMENT | - |
dc.subject.keywordPlus | CADHERIN | - |
dc.subject.keywordPlus | VEGFR-2 | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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