Mitigation of indomethacin-induced gastrointestinal damages in fat-1 transgenic mice via gate-keeper action of omega-3-polyunsaturated fatty acids
DC Field | Value | Language |
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dc.contributor.author | Han, Young-Min | - |
dc.contributor.author | Park, Jong-Min | - |
dc.contributor.author | Kang, Jing X. | - |
dc.contributor.author | Cha, Ji-Young | - |
dc.contributor.author | Lee, Ho-Jae | - |
dc.contributor.author | Jeong, Migeyong | - |
dc.contributor.author | Go, Eun-Jin | - |
dc.contributor.author | Hahm, Ki Baik | - |
dc.date.available | 2020-02-28T00:43:37Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-09-23 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7879 | - |
dc.description.abstract | Non-steroidal anti-inflammatory drugs (NSAIDs) damage the gastrointestinal (GI) epithelial cell membranes by inducing several signals through lipid raft organization after membrane incorporation, whereas omega-3 polyunsaturated fatty acids (PUFAs) relieve inflammation, reduce oxidative stress, and provide cytoprotection, consequent to lipid raft disorganization. Therefore, we hypothesized that omega-3 PUFAs can protect the GI from NSAID-induced damages by initiating the gatekeeper action of cell membranes, subsequent to anti-inflammatory and anti-oxidative actions. Administration of indomethacin (IND) leads to the formation of lipid rafts and activation of caveolin-1; however, no such observations were made upon co-administration of eicosapentaenoic acid (EPA) and IND. In addition, the EPA-induced lipid raft disorganization, caveolin-1 inactivation, and cellular cytotoxicity were inhibited when target cells were knocked-out using G-protein coupled receptor 120 (GPR 120). EPA significantly attenuated IND-induced oxidative damage and apoptosis. IND administration induced significant ulceration, bleeding, and oedema in the stomach or small intestine of wild-type (WT) mice; however, such severe damages to the GI significantly decreased in fat-1 transgenic (TG) mice (P < 0.001), which exhibited decreased cyclooxygenase-2 expression and apoptosis, decreased interleukin-1 beta and FAS concentrations, and increased heme oxygenase-1 concentration. Our study indicates that the gatekeeper function of omega-3 PUFAs improves GI safety when administered with NSAID. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.subject | NONSTEROIDAL ANTIINFLAMMATORY DRUGS | - |
dc.subject | GASTRIC-MUCOSAL INJURY | - |
dc.subject | FISH-OIL | - |
dc.subject | LIPID RAFTS | - |
dc.subject | NSAIDS | - |
dc.subject | RATS | - |
dc.subject | PHOSPHOLIPIDS | - |
dc.subject | MEMBRANES | - |
dc.subject | INSIGHT | - |
dc.subject | PHOSPHATIDYLCHOLINE | - |
dc.title | Mitigation of indomethacin-induced gastrointestinal damages in fat-1 transgenic mice via gate-keeper action of omega-3-polyunsaturated fatty acids | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000384067500001 | - |
dc.identifier.doi | 10.1038/srep33992 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6 | - |
dc.identifier.scopusid | 2-s2.0-84989260699 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Cha, Ji-Young | - |
dc.contributor.affiliatedAuthor | Lee, Ho-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NONSTEROIDAL ANTIINFLAMMATORY DRUGS | - |
dc.subject.keywordPlus | GASTRIC-MUCOSAL INJURY | - |
dc.subject.keywordPlus | FISH-OIL | - |
dc.subject.keywordPlus | LIPID RAFTS | - |
dc.subject.keywordPlus | NSAIDS | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordPlus | PHOSPHOLIPIDS | - |
dc.subject.keywordPlus | MEMBRANES | - |
dc.subject.keywordPlus | INSIGHT | - |
dc.subject.keywordPlus | PHOSPHATIDYLCHOLINE | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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