Cannabidiol induces osteoblast differentiation via angiopoietin1 and p38 MAPK
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, M.-A. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Park, S.-H. | - |
dc.date.available | 2020-11-18T00:40:16Z | - |
dc.date.created | 2020-07-20 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1520-4081 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78950 | - |
dc.description.abstract | In this study, we report the potential of cannabidiol, one of the major cannabis constituents, for enhancing osteoblastic differentiation in U2OS and MG-63 cells. Cannabidiol increased the expression of Angiopoietin1 and the enzyme activity of alkaline phosphatase in U2OS and MG-63. Invasion and migration assay results indicated that the cell mobility was activated by cannabidiol in U2OS and MG-63. Western blotting analysis showed that the expression of tight junction related proteins such as Claudin1, Claudin4, Occuludin1, and ZO1 was increased by cannabidiol in U2OS and MG-63. Alizarin Red S staining analysis showed that calcium deposition and mineralization was enhanced by cannabidiol in U2OS and MG-63. Western blotting analysis indicated that the expression of osteoblast differentiation related proteins such as distal-less homeobox 5, bone sialoprotein, osteocalcin, type I collagen, Runt-related transcription factor 2 (RUNX2), osterix (OSX), and alkaline phosphatase was time dependently upregulated by cannabidiol in U2OS and MG-63. Mechanistically, cannabidiol-regulated osteoblastic differentiation in U2OS and MG-63 by strengthen the protein-protein interaction among RUNX2, OSX, or the phosphorylated p38 mitogen-activated protein kinase (MAPK). In conclusion, cannabidiol increased Angiopoietin1 expression and p38 MAPK activation for osteoblastic differentiation in U2OS and MG-63 suggesting that cannabidiol might provide a novel therapeutic option for the bone regeneration. © 2020 Wiley Periodicals LLC. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | Environmental Toxicology | - |
dc.title | Cannabidiol induces osteoblast differentiation via angiopoietin1 and p38 MAPK | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000586008300004 | - |
dc.identifier.doi | 10.1002/tox.22996 | - |
dc.identifier.bibliographicCitation | Environmental Toxicology, v.35, no.12, pp.1318 - 1325 | - |
dc.identifier.scopusid | 2-s2.0-85087769001 | - |
dc.citation.endPage | 1325 | - |
dc.citation.startPage | 1318 | - |
dc.citation.title | Environmental Toxicology | - |
dc.citation.volume | 35 | - |
dc.citation.number | 12 | - |
dc.contributor.affiliatedAuthor | Kang, M.-A. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | angiopoietin1 | - |
dc.subject.keywordAuthor | cannabidiol | - |
dc.subject.keywordAuthor | osteoblastic differentiation | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordPlus | Bone | - |
dc.subject.keywordPlus | Enzyme activity | - |
dc.subject.keywordPlus | Tissue regeneration | - |
dc.subject.keywordPlus | Transcription | - |
dc.subject.keywordPlus | ALkaline phosphatase | - |
dc.subject.keywordPlus | Bone regeneration | - |
dc.subject.keywordPlus | Bone sialoproteins | - |
dc.subject.keywordPlus | Calcium deposition | - |
dc.subject.keywordPlus | Osteoblast differentiation | - |
dc.subject.keywordPlus | Osteoblastic differentiation | - |
dc.subject.keywordPlus | P38 mitogen-activated protein kinase | - |
dc.subject.keywordPlus | Protein-protein interactions | - |
dc.subject.keywordPlus | Phosphatases | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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