Enhanced Intestinal Immune Response in Mice after Oral Administration of Korea Red Ginseng-Derived Polysaccharide
DC Field | Value | Language |
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dc.contributor.author | Park, Do Hwi | - |
dc.contributor.author | Han, Byungcheol | - |
dc.contributor.author | Shin, Myoung-Sook | - |
dc.contributor.author | Hwang, Gwi Seo | - |
dc.date.available | 2020-11-18T00:40:44Z | - |
dc.date.created | 2020-10-21 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78961 | - |
dc.description.abstract | (1) Background: The immunostimulatory role of the polysaccharide fraction (KRG-P) of Korea red ginseng (KRG) was studied in cells. However, its immunomodulatory activity is unknown. Therefore, we investigated the chemical properties of KRG-P and its intestinal immune responses in vitro and in vivo. (2) Methods: KRG-P monosaccharide composition and molecular weight were determined using high-performance liquid and size-exclusion chromatography systems. Immunoglobulin A (IgA) and α-defensin-1 transcript levels were measured using a SYBR Green qRT-PCR; defensin-1, Granulocyte-macrophage colony-stimulating factor (GM-CSF), and IgA protein levels were determined using Western blotting and ELISA kits. (3) Results: The molecular weight of KRG-P was estimated to be 106 kDa, and it contained neutral sugar (74.3%), uronic acid (24.6%), and proteins (1%). In vitro studies of intestinal immunomodulatory activity of KRG-P indicated that GM-CSF and IgA levels increased in Peyer's patch cells to higher levels than those obtained with KRG and induced bone marrow cell proliferation. In in vivo study, oral KRG-P administration to mice upregulated the expression of α-defensin-1 and IgA in the small intestinal tissue and that of secreted IgA in the feces. (4) Conclusions: KRG-P contributed to the modulation of intestinal immunity and maintenance of intestinal homeostasis against intestinal infection. © 2020 by the authors. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Polymers | - |
dc.title | Enhanced Intestinal Immune Response in Mice after Oral Administration of Korea Red Ginseng-Derived Polysaccharide | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000585297800001 | - |
dc.identifier.doi | 10.3390/POLYM12102186 | - |
dc.identifier.bibliographicCitation | Polymers, v.12, no.10 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85092432101 | - |
dc.citation.title | Polymers | - |
dc.citation.volume | 12 | - |
dc.citation.number | 10 | - |
dc.contributor.affiliatedAuthor | Park, Do Hwi | - |
dc.contributor.affiliatedAuthor | Shin, Myoung-Sook | - |
dc.contributor.affiliatedAuthor | Hwang, Gwi Seo | - |
dc.identifier.url | https://www.mdpi.com/2073-4360/12/10/2186 | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | IgA | - |
dc.subject.keywordAuthor | Korea red ginseng polysaccharide | - |
dc.subject.keywordAuthor | Peyer&apos | - |
dc.subject.keywordAuthor | s patch | - |
dc.subject.keywordAuthor | α-defensin-1 | - |
dc.subject.keywordPlus | Bone | - |
dc.subject.keywordPlus | Cell proliferation | - |
dc.subject.keywordPlus | Liquid chromatography | - |
dc.subject.keywordPlus | Macrophages | - |
dc.subject.keywordPlus | Mammals | - |
dc.subject.keywordPlus | Molecular weight | - |
dc.subject.keywordPlus | Proteins | - |
dc.subject.keywordPlus | Size exclusion chromatography | - |
dc.subject.keywordPlus | Bone marrow cells | - |
dc.subject.keywordPlus | Granulocyte-macrophage colony stimulating factors | - |
dc.subject.keywordPlus | Immunoglobulin a (IgA) | - |
dc.subject.keywordPlus | Immunomodulatory activities | - |
dc.subject.keywordPlus | Monosaccharide composition | - |
dc.subject.keywordPlus | Oral administration | - |
dc.subject.keywordPlus | Transcript level | - |
dc.subject.keywordPlus | Western blotting | - |
dc.subject.keywordPlus | Immune system | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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