Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line
DC Field | Value | Language |
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dc.contributor.author | Lee, D. | - |
dc.contributor.author | Choi, H.G. | - |
dc.contributor.author | Hwang, J.H. | - |
dc.contributor.author | Shim, S.H. | - |
dc.contributor.author | Kang, K.S. | - |
dc.date.available | 2020-12-09T00:40:36Z | - |
dc.date.created | 2020-11-20 | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79216 | - |
dc.description.abstract | Excessive glutamate damages neuronal cells via the accumulation of intracellular reactive oxygen species (ROS), calcium ion (Ca2+) influx, depolarization of mitochondrial membrane potential, and apoptosis, which may result in the development of chronic neurodegenerative diseases. In this study, we evaluated the effects of 4,6′-anhydrooxysporidinone isolated from endophytic fungus Fusarium lateritium SSF2 on glutamate-induced cytotoxicity, accumulation of intracellular ROS, increases in superoxide anion production, Ca2+, depolarization of mitochondrial membrane potential, and apoptotic cell death in hippocampal HT22 cells. 2′,7′-Dichlorofluorescin diacetate (H2DCFDA) staining was used to determine the intracellular reactive oxygen species concentration and dihydroethidine (DHE) staining was used to determine the superoxide radical. Expression of the nuclear factor-erythroid-2–related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was analyzed by Western blot. Fluo-4 staining was used to determine the intracellular Ca2+ levels. In order to explore mitochondrial membrane potential, tetramethylrhodamine methyl ester (TMRM) staining was used. Apoptotic cell death was evaluated using Annexin-V/propidium iodide (PI) staining and TUNEL staining. Expression of the cytochrome c release and cleaved caspase-9,-3 was analyzed by Western blot. Here, we were able to isolate 4,6′-anhydrooxysporidinone from endophytic fungus, Fusarium lateritium SSF2, which was shown to protect HT22 cells from glutamate-induced cytotoxicity, accumulation of intracellular ROS, increases in superoxide anion production, Ca2+, and depolarization of mitochondrial membrane potential. In addition, 4,6′-anhydrooxysporidinone enhanced the expressions of Nrf2 and HO-1. It also inhibited the apoptotic cell death through the inhibition of cytochrome c release and cleaved caspase-9,-3 in glutamate-treated HT22 cells. Therefore, our results provide ample evidence of the neuroprotective properties of 4,6′-anhydrooxysporidinone. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.relation.isPartOf | Antioxidants | - |
dc.title | Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000592981900001 | - |
dc.identifier.doi | 10.3390/antiox9111115 | - |
dc.identifier.bibliographicCitation | Antioxidants, v.9, no.11, pp.1 - 15 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85095982219 | - |
dc.citation.endPage | 15 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Antioxidants | - |
dc.citation.volume | 9 | - |
dc.citation.number | 11 | - |
dc.contributor.affiliatedAuthor | Lee, D. | - |
dc.contributor.affiliatedAuthor | Hwang, J.H. | - |
dc.contributor.affiliatedAuthor | Kang, K.S. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Ca2+ | - |
dc.subject.keywordAuthor | Fusarium lateritium SSF2 | - |
dc.subject.keywordAuthor | Glutamate | - |
dc.subject.keywordAuthor | HT22 cells | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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