Simultaneous determination of N-hydroxymethyl-N-methylformamide, N-methylformamide and N-acetyl-S-(N-methylcarbamoyl)cystein in urine samples from workers exposed to N,N-dimethylformamide by liquid chromatography-tandem mass spectrometry
DC Field | Value | Language |
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dc.contributor.author | Sohn, JH | - |
dc.contributor.author | Han, MJ | - |
dc.contributor.author | Lee, MY | - |
dc.contributor.author | Kang, SK | - |
dc.contributor.author | Yang, JS | - |
dc.date.available | 2020-12-15T02:40:27Z | - |
dc.date.created | 2020-12-15 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79285 | - |
dc.description.abstract | N-Hydroxymethyl-N-methylformamide (HMMF) and N-methylformamide (NMF) in urine samples from workers exposed to N,N-dimethylformamide (DMF) cannot be distinguished by a gas chromatographic method because HMMF is converted to NMF at the injection port of gas chromatography (GC). Total NMF (HMMF + NMF) has been measured instead. Also, the determination of N-acetyl-S(N-methylcarbamoyl)cystein (AMCC), which is supposed to be related to the toxicity of DMF, needs multiple treatments to convert to a volatile compound before GC analysis. There is no previous report of a simultaneous determination of three major metabolites of DMF in urine. The aim of this study is to develop a simple and selective method for the determination of DMF metabolite in urine. By using a liquid chromatography-tandem mass spectrometry, we can directly distinguish these three major metabolites of DMF in a single run. The diluted urine samples were analyzed on Capcell Pak MF SG80 column with the mobile phase of methanol in 2 mM formic acid (10:90, v/v). The analytes were detected by an electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring mode. The standard curves were linear (r>0.999) over the concentration ranges of 0.004-8 mug/mL. The precision and accuracy of quality control samples for inter-batch (n = 6) analyses were in the range of 1.3-9.8% and 94.7-116.8, respectively. The sum of each HMMF and NMF concentration determined by LC-MS/MS method shows high correlation (r = 0.9927 with the slope of 1.0415, p < 0.0001) with NMF included HMMF concentration determined by GC method for 13 urine samples taken from workers exposed to DMF. The excretion ratio of HMMF:NMF:AMCC is approximately 4: 1: 1 in molar concentration. (C) 2004 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.title | Simultaneous determination of N-hydroxymethyl-N-methylformamide, N-methylformamide and N-acetyl-S-(N-methylcarbamoyl)cystein in urine samples from workers exposed to N,N-dimethylformamide by liquid chromatography-tandem mass spectrometry | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000227014200020 | - |
dc.identifier.doi | 10.1016/j.jpba.2004.10.001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.37, no.1, pp.165 - 170 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-12344257814 | - |
dc.citation.endPage | 170 | - |
dc.citation.startPage | 165 | - |
dc.citation.title | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.citation.volume | 37 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kang, SK | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | N,N-dimethylformamide | - |
dc.subject.keywordAuthor | N-hydroxymethyl-N-methylformamide | - |
dc.subject.keywordAuthor | N-methylformamide | - |
dc.subject.keywordAuthor | N-acetyl-S-(N-methylcarbamoyl)cystein | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordPlus | OCCUPATIONAL-EXPOSURE | - |
dc.subject.keywordPlus | DIMETHYLFORMAMIDE | - |
dc.subject.keywordPlus | HEPATOTOXICITY | - |
dc.subject.keywordPlus | METABOLITES | - |
dc.subject.keywordPlus | HUMANS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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