Resolvin D3 Promotes Inflammatory Resolution, Neuroprotection, and Functional Recovery After Spinal Cord Injury
DC Field | Value | Language |
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dc.contributor.author | Kim, J. | - |
dc.contributor.author | Joshi, H.P. | - |
dc.contributor.author | Sheen, S.H. | - |
dc.contributor.author | Kim, K.-T. | - |
dc.contributor.author | Kyung, J.W. | - |
dc.contributor.author | Choi, H. | - |
dc.contributor.author | Kim, Y.W. | - |
dc.contributor.author | Kwon, S.Y. | - |
dc.contributor.author | Roh, E.J. | - |
dc.contributor.author | Choi, U.Y. | - |
dc.contributor.author | Sohn, S. | - |
dc.contributor.author | Kim, Y.H. | - |
dc.contributor.author | Park, C.-K. | - |
dc.contributor.author | Kumar, H. | - |
dc.contributor.author | Han, I.-B. | - |
dc.date.available | 2020-12-23T01:40:08Z | - |
dc.date.created | 2020-09-29 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 0893-7648 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79394 | - |
dc.description.abstract | Resolvins, a new family from the endogenous specialized pro-resolving mediators (SPMs), promote the resolution of the inflammatory response. Resolvin D3 (RvD3), a docosahexaenoic acid (DHA) product, has been known to suppress the inflammatory response. However, the anti-inflammatory and neuroprotective effects of RvD3 are not known in a model of spinal cord injury (SCI). Here, we investigated the anti-inflammatory and neuroprotective effect of RvD3 in a mouse model of SCI. Processes associated with anti-inflammation and angiogenesis were studied in RAW 264.7 cells and the human brain endothelial cell line hCMEC/D3, respectively. Additionally, female C57BL/6 mice were subjected to moderate compression SCI (20-g weight compression for 1 min) followed by intrathecal injection of vehicle or RvD3 (1 μg/20 μL) at 1 h post-SCI. RvD3 decreased the lipopolysaccharide (LPS)-induced production of inflammatory mediators and nitric oxide (NO) in RAW 264.7 cells and promoted an angiogenic effect in the hCMEC/D3 cell line. Treatment with RvD3 improved locomotor recovery and reduced thermal hyperalgesia in SCI mice compared with vehicle treatment at 14 days post-SCI. Remarkably, RvD3-treated mice exhibited reduced expression of inflammatory cytokines (TNF-α, IL6, IL1β) and chemokines (CCL2, CCL3). Also, RvD3-treated mice exhibited increased expression of tight junction proteins such as zonula occludens (ZO)-1 and occludin. Furthermore, immunohistochemistry showed a decreased level of gliosis (GFAP, Iba-1) and neuroinflammation (CD68, TGF-β) and enhanced neuroprotection. These data provide evidence that intrathecal injection of RvD3 represents a promising therapeutic strategy to promote inflammatory resolution, neuroprotection, and neurological functional recovery following SCI. © 2020, Springer Science+Business Media, LLC, part of Springer Nature. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Springer | - |
dc.relation.isPartOf | Molecular Neurobiology | - |
dc.title | Resolvin D3 Promotes Inflammatory Resolution, Neuroprotection, and Functional Recovery After Spinal Cord Injury | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000572054100002 | - |
dc.identifier.doi | 10.1007/s12035-020-02118-7 | - |
dc.identifier.bibliographicCitation | Molecular Neurobiology, v.58, no.1, pp.424 - 438 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85091352976 | - |
dc.citation.endPage | 438 | - |
dc.citation.startPage | 424 | - |
dc.citation.title | Molecular Neurobiology | - |
dc.citation.volume | 58 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Y.H. | - |
dc.contributor.affiliatedAuthor | Park, C.-K. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Inflammatory response | - |
dc.subject.keywordAuthor | Pain | - |
dc.subject.keywordAuthor | Resolvin D3 | - |
dc.subject.keywordAuthor | Resolvins | - |
dc.subject.keywordAuthor | Secondary injury | - |
dc.subject.keywordAuthor | Spinal cord injury | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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