All-trans Retinoic Acid Induces Expression and Secretion of Carboxypeptidase D in THP-1 Cells
DC Field | Value | Language |
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dc.contributor.author | Hang Thi Thu Nguyen | - |
dc.contributor.author | 김재영 | - |
dc.date.available | 2021-01-08T00:40:21Z | - |
dc.date.created | 2021-01-08 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1738-3226 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79661 | - |
dc.description.abstract | Carboxypeptidase D (CPD) is a zinc-dependent protease, which is highly expressed in macrophages, and is thought to participate in inflammatory processes. In the present study, we investigated the possible regulatory effect of all-trans retinoic acid (ATRA), which is an active form of vitamin A and plays a critical regulatory role in both the innate and adaptive immunity, on CPD expression and secretion in human monocytic THP-1 cells. CPD mRNA expression first increased, from a concentration as low as 10 nM ATRA to a maximum level of expression, at 1 μM. ATRA enhanced intracellular CPD expression in a time- and concentration-dependent manner but did not affect cell surface CPD expression. Interestingly, 9-cis-RA did not affect CPD expression. Additionally, an experiment with RAR/RXR selective agonist or antagonists demonstrated that ATRA-induced enhancement of CPD expression was RAR/RXR dependent. ATRA also enhanced CPD secretion from THP-1 cells; however, this enhancement was RAR/RXR-independent. The anti-inflammatory agent dexamethasone reversed ATRA-induced enhancement of CPD expression and secretion. Our results suggest ATRA exerts regulatory effects on expression and secretion of CPD in human monocytes, and ATRA-induced CPD secretion may be associated with inflammatory response. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한의생명과학회 | - |
dc.relation.isPartOf | 대한의생명과학회지 | - |
dc.title | All-trans Retinoic Acid Induces Expression and Secretion of Carboxypeptidase D in THP-1 Cells | - |
dc.title.alternative | All-trans Retinoic Acid Induces Expression and Secretion of Carboxypeptidase D in THP-1 Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 대한의생명과학회지, v.26, no.4, pp.256 - 266 | - |
dc.identifier.kciid | ART002673771 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 266 | - |
dc.citation.startPage | 256 | - |
dc.citation.title | 대한의생명과학회지 | - |
dc.citation.volume | 26 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Hang Thi Thu Nguyen | - |
dc.contributor.affiliatedAuthor | 김재영 | - |
dc.subject.keywordAuthor | All-trans retinoic acid | - |
dc.subject.keywordAuthor | Carboxypeptidase D | - |
dc.subject.keywordAuthor | THP-1 | - |
dc.subject.keywordAuthor | Monocytes | - |
dc.subject.keywordAuthor | Vitamin A | - |
dc.subject.keywordAuthor | Secretion | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.description.journalRegisteredClass | kci | - |
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