Ecofriendly synthesis of silver nanoparticles by Terrabacter humi sp. nov. and their antibacterial application against antibiotic-resistant pathogens
DC Field | Value | Language |
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dc.contributor.author | Akter, S. | - |
dc.contributor.author | Lee, S.-Y. | - |
dc.contributor.author | Siddiqi, M.Z. | - |
dc.contributor.author | Balusamy, S.R. | - |
dc.contributor.author | Ashrafudoulla, M. | - |
dc.contributor.author | Rupa, E.J. | - |
dc.contributor.author | Huq, M.A. | - |
dc.date.available | 2021-01-13T01:40:29Z | - |
dc.date.created | 2021-01-04 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79700 | - |
dc.description.abstract | It is essential to develop and discover alternative eco-friendly antibacterial agents due to the emergence of multi-drug-resistant microorganisms. In this study, we isolated and characterized a novel bacterium named Terrabacter humi MAHUQ-38T, utilized for the eco-friendly synthesis of silver nanoparticles (AgNPs) and the synthesized AgNPs were used to control multi-drug-resistant microorganisms. The novel strain was Gram stain positive, strictly aerobic, milky white colored, rod shaped and non-motile. The optimal growth temperature, pH and NaCl concentration were 30◦ C, 6.5 and 0%, respectively. Based on 16S rRNA gene sequence, strain MAHUQ-38T belongs to the genus Terrabacter and is most closely related to several Terrabacter type strains (98.2%–98.8%). Terrabacter humi MAHUQ-38T had a genome of 5,156,829 bp long (19 contigs) with 4555 protein-coding genes, 48 tRNA and 5 rRNA genes. The culture supernatant of strain MAHUQ-38T was used for the eco-friendly and facile synthesis of AgNPs. The transmission electron microscopy (TEM) image showed the spherical shape of AgNPs with a size of 6 to 24 nm, and the Fourier transform infrared (FTIR) analysis revealed the functional groups responsible for the synthesis of AgNPs. The synthesized AgNPs exhibited strong anti-bacterial activity against multi-drug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 6.25/50 and 12.5/50 µg/mL, respectively. The AgNPs altered the cell morphology and damaged the cell membrane of pathogens. This study encourages the use of Terrabacter humi for the ecofriendly synthesis of AgNPs to control multi-drug-resistant microorganisms. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | International Journal of Molecular Sciences | - |
dc.title | Ecofriendly synthesis of silver nanoparticles by Terrabacter humi sp. nov. and their antibacterial application against antibiotic-resistant pathogens | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000602955600001 | - |
dc.identifier.doi | 10.3390/ijms21249746 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.21, no.24, pp.1 - 19 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85098326367 | - |
dc.citation.endPage | 19 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 21 | - |
dc.citation.number | 24 | - |
dc.contributor.affiliatedAuthor | Akter, S. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | AgNPs | - |
dc.subject.keywordAuthor | Eco-friendly synthesis | - |
dc.subject.keywordAuthor | Multi-drug-resistant microorganisms | - |
dc.subject.keywordAuthor | Terrabacter humi MAHUQ-38T | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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