Human skeletal muscle metabolic responses to 6 days of high-fat overfeeding are associated with dietary n-3PUFA content and muscle oxidative capacity
DC Field | Value | Language |
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dc.contributor.author | Wardle, Sophie L. | - |
dc.contributor.author | Macnaughton, Lindsay S. | - |
dc.contributor.author | McGlory, Chris | - |
dc.contributor.author | Witard, Oliver C. | - |
dc.contributor.author | Dick, James R. | - |
dc.contributor.author | Whitfield, Philip D. | - |
dc.contributor.author | Ferrando, Arny A. | - |
dc.contributor.author | Wolfe, Robert R. | - |
dc.contributor.author | Kim, Il-Young | - |
dc.contributor.author | Hamilton, D. Lee | - |
dc.contributor.author | Moran, Colin N. | - |
dc.contributor.author | Tipton, Kevin D. | - |
dc.contributor.author | Galloway, Stuart D. R. | - |
dc.date.available | 2021-02-03T09:40:03Z | - |
dc.date.created | 2021-02-03 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 2051-817X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79884 | - |
dc.description.abstract | Understanding human physiological responses to high-fat energy excess (HFEE) may help combat the development of metabolic disease. We aimed to investigate the impact of manipulating the n-3PUFA content of HFEE diets on whole-body and skeletal muscle markers of insulin sensitivity. Twenty healthy males were overfed (150% energy, 60% fat, 25% carbohydrate, 15% protein) for 6 d. One group (n = 10) received 10% of fat intake as n-3PUFA rich fish oil (HF-FO), and the other group consumed a mix of fats (HF-C). Oral glucose tolerance tests with stable isotope tracer infusions were conducted before, and following, HFEE, with muscle biopsies obtained in basal and insulin-stimulated states for measurement of membrane phospholipids, ceramides, mitochondrial enzyme activities, and PKB and AMPK alpha 2 activity. Insulin sensitivity and glucose disposal did not change following HFEE, irrespective of group. Skeletal muscle ceramide content increased following HFEE (8.5 +/- 1.2 to 12.1 +/- 1.7 nmol/mg, p = .03), irrespective of group. No change in mitochondrial enzyme activity was observed following HFEE, but citrate synthase activity was inversely associated with the increase in the ceramide content (r=-0.52,p = .048). A time by group interaction was observed for PKB activity (p = .003), with increased activity following HFEE in HF-C (4.5 +/- 13.0mU/mg) and decreased activity in HF-FO (-10.1 +/- 20.7 mU/mg) following HFEE. Basal AMPK alpha 2 activity increased in HF-FO (4.1 +/- 0.6 to 5.3 +/- 0.7mU/mg, p = .049), but did not change in HF-C (4.6 +/- 0.7 to 3.8 +/- 0.9mU/mg) following HFEE. We conclude that early skeletal muscle signaling responses to HFEE appear to be modified by dietary n-3PUFA content, but the potential impact on future development of metabolic disease needs exploring. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | PHYSIOLOGICAL REPORTS | - |
dc.title | Human skeletal muscle metabolic responses to 6 days of high-fat overfeeding are associated with dietary n-3PUFA content and muscle oxidative capacity | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000567320300001 | - |
dc.identifier.doi | 10.14814/phy2.14529 | - |
dc.identifier.bibliographicCitation | PHYSIOLOGICAL REPORTS, v.8, no.16 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85089885121 | - |
dc.citation.title | PHYSIOLOGICAL REPORTS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 16 | - |
dc.contributor.affiliatedAuthor | Kim, Il-Young | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | exercise | - |
dc.subject.keywordAuthor | fish oil | - |
dc.subject.keywordAuthor | insulin resistance | - |
dc.subject.keywordAuthor | omega-3 | - |
dc.subject.keywordAuthor | overfeeding | - |
dc.subject.keywordAuthor | type 2 diabetes | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | SHORT-TERM | - |
dc.subject.keywordPlus | DIABETES-MELLITUS | - |
dc.subject.keywordPlus | WEIGHT-GAIN | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | HEALTHY | - |
dc.subject.keywordPlus | ACIDS | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordPlus | LIPOPROTEINS | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scopus | - |
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