Notch1 Has an Important Role in beta-Cell Mass Determination and Development of Diabetes
DC Field | Value | Language |
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dc.contributor.author | Eom, Young Sil | - |
dc.contributor.author | Gwon, A-Ryeong | - |
dc.contributor.author | Kwak, Kyung Min | - |
dc.contributor.author | Youn, Jin-Young | - |
dc.contributor.author | Park, Heekyoung | - |
dc.contributor.author | Kim, Kwang-Won | - |
dc.contributor.author | Kim, Byung-Joon | - |
dc.date.available | 2021-03-29T00:40:49Z | - |
dc.date.created | 2020-08-27 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 2233-6079 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80564 | - |
dc.description.abstract | Background: Notch signaling pathway plays an important role in regulating pancreatic endocrine and exocrine cell fate during pancreas development. Notch signaling is also expressed in adult pancreas. There are few studies on the effect of Notch on adult pancreas. Here, we investigated the role of Notch in islet mass and glucose homeostasis in adult pancreas using Notch1 antisense transgenic (NAS). Methods: Western blot analysis was performed for the liver of 8-week-old male NAS mice. We also conducted an intraperitoneal glucose tolerance test (IPGTT) and intraperitoneal insulin tolerance test in 8-week-old male NAS mice and male C57BL/6 mice (control). Morphologic observation of pancreatic islet and β-cell was conducted in two groups. Insulin secretion capacity in islets was measured by glucose-stimulated insulin secretion (GSIS) and perifusion. Results: NAS mice showed higher glucose levels and lower insulin secretion in IPGTT than the control mice. There was no significant difference in insulin resistance. Total islet and β-cell masses were decreased in NAS mice. The number of large islets (≥250 μm) decreased while that of small islets (<250 μm) increased. Reduced insulin secretion was observed in GSIS and perifusion. Neurogenin3, neurogenic differentiation, and MAF bZIP transcription factor A levels increased in NAS mice. Conclusion: Our study provides that Notch1 inhibition decreased insulin secretion and decreased islet and β-cell masses. It is thought that Notch1 inhibition suppresses islet proliferation and induces differentiation of small islets. In conclusion, Notch signaling pathway may play an important role in β-cell mass determination and diabetes. © 2020 Korean Diabetes Association | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Korean Diabetes Association | - |
dc.relation.isPartOf | Diabetes and Metabolism Journal | - |
dc.title | Notch1 Has an Important Role in beta-Cell Mass Determination and Development of Diabetes | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000625390100009 | - |
dc.identifier.doi | 10.4093/dmj.2019.0160 | - |
dc.identifier.bibliographicCitation | Diabetes and Metabolism Journal, v.45, no.1, pp.86 - 96 | - |
dc.identifier.kciid | ART002680732 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85089499035 | - |
dc.citation.endPage | 96 | - |
dc.citation.startPage | 86 | - |
dc.citation.title | Diabetes and Metabolism Journal | - |
dc.citation.volume | 45 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Eom, Young Sil | - |
dc.contributor.affiliatedAuthor | Gwon, A-Ryeong | - |
dc.contributor.affiliatedAuthor | Kwak, Kyung Min | - |
dc.contributor.affiliatedAuthor | Youn, Jin-Young | - |
dc.contributor.affiliatedAuthor | Park, Heekyoung | - |
dc.contributor.affiliatedAuthor | Kim, Kwang-Won | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Joon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Diabetes mellitus | - |
dc.subject.keywordAuthor | Glucose | - |
dc.subject.keywordAuthor | Insulin secretion | - |
dc.subject.keywordAuthor | Insulin-secreting cells | - |
dc.subject.keywordAuthor | Notch | - |
dc.subject.keywordAuthor | Receptors | - |
dc.subject.keywordPlus | basic leucine zipper transcription factor | - |
dc.subject.keywordPlus | glucose | - |
dc.subject.keywordPlus | insulin | - |
dc.subject.keywordPlus | neurogenin 3 | - |
dc.subject.keywordPlus | Notch1 receptor | - |
dc.subject.keywordPlus | transcription factor PDX 1 | - |
dc.subject.keywordPlus | adult | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | comparative study | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | diabetes mellitus | - |
dc.subject.keywordPlus | genotype | - |
dc.subject.keywordPlus | glucose blood level | - |
dc.subject.keywordPlus | glucose homeostasis | - |
dc.subject.keywordPlus | glucose tolerance test | - |
dc.subject.keywordPlus | in vitro study | - |
dc.subject.keywordPlus | insulin blood level | - |
dc.subject.keywordPlus | insulin release | - |
dc.subject.keywordPlus | insulin resistance | - |
dc.subject.keywordPlus | insulin tolerance test | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | pancreas islet | - |
dc.subject.keywordPlus | pancreas islet beta cell | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | Western blotting | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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