Bruton's agammaglobulinemia tyrosine kinase (Btk) regulates TPA-induced breast cancer cell invasion via PLC gamma 2/PKC beta/NF-kappa B/AP-1-dependent matrix metalloproteinase-9 activation
DC Field | Value | Language |
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dc.contributor.author | Kim, Jeong-Mi | - |
dc.contributor.author | Park, Jinny | - |
dc.contributor.author | Noh, Eun-Mi | - |
dc.contributor.author | Song, Hyun-Kyung | - |
dc.contributor.author | Kang, Sang Yull | - |
dc.contributor.author | Jung, Sung Hoo | - |
dc.contributor.author | Kim, Jong-Suk | - |
dc.contributor.author | Park, Byung-Hyun | - |
dc.contributor.author | Lee, Young-Rae | - |
dc.contributor.author | Youn, Hyun Jo | - |
dc.date.available | 2021-04-02T01:40:09Z | - |
dc.date.created | 2021-04-02 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1021-335X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80618 | - |
dc.description.abstract | Bruton's agammaglobulinemia tyrosine kinase (BTK) is an important cytoplasmic tyrosine kinase involved in B-lymphocyte development, differentiation, and signaling. Activated protein kinase C (PKC), in turn, induces the activation of mitogen-activated protein kinase (MAPK) signaling, which promotes cell proliferation, viability, apoptosis, and metastasis. This effect is associated with nuclear factor-kappa B (NF-kappa B) activation, suggesting an anti-metastatic effect of BTK inhibitors on MCF-7 cells that leads to the downregulation of matrix metalloproteinase (MMP)-9 expression. However, the effect of BTK on breast cancer metastasis is unknown. In this study, the anti-metastatic activity of BTK inhibitors was examined in MCF-7 cells focusing on MMP-9 expression in 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated MCF-7 cells. The expression and activity of MMP-9 in MCF-7 cells were investigated using quantitative polymerase chain reaction analysis, western blotting, and zymography. Cell invasion and migration were investigated using the Matrigel invasion and cell migration assays. BTK inhibitors [ibrutinib (10 mu M), CNX-774 (10 mu M)] significantly attenuated TPA-induced cell invasion and migration in MCF-7 cells and inhibited the activation of the phospholipase C gamma 2/PKC beta signaling pathways. In addition, small interfering RNA specific for BTK suppressed MMP-9 expression and cell metastasis. Collectively, results of the present study indicated that BTK suppressed TPA-induced MMP-9 expression and cell invasion/migration by activating the MAPK or I kappa B kinase/NF-kappa B/activator protein-1 pathway. The results clarify the mechanism of action of BTK in cancer cell metastasis by regulating MMP-9 expression in MCF-7 cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.relation.isPartOf | ONCOLOGY REPORTS | - |
dc.title | Bruton's agammaglobulinemia tyrosine kinase (Btk) regulates TPA-induced breast cancer cell invasion via PLC gamma 2/PKC beta/NF-kappa B/AP-1-dependent matrix metalloproteinase-9 activation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000629630300001 | - |
dc.identifier.doi | 10.3892/or.2021.8007 | - |
dc.identifier.bibliographicCitation | ONCOLOGY REPORTS, v.45, no.5 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85103571882 | - |
dc.citation.title | ONCOLOGY REPORTS | - |
dc.citation.volume | 45 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Park, Jinny | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Bruton& | - |
dc.subject.keywordAuthor | apos | - |
dc.subject.keywordAuthor | s agammaglobulinemia tyrosine kinase | - |
dc.subject.keywordAuthor | matrix metalloproteinase-9 | - |
dc.subject.keywordAuthor | protein kinase C | - |
dc.subject.keywordAuthor | MCF-7 cells | - |
dc.subject.keywordAuthor | neoplasm invasiveness | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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