6,8-Diprenylorobol induces apoptosis in human colon cancer cells via activation of intracellular reactive oxygen species and p53
DC Field | Value | Language |
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dc.contributor.author | Choi, Y.J. | - |
dc.contributor.author | Lee, J. | - |
dc.contributor.author | Ha, S.H. | - |
dc.contributor.author | Lee, H.K. | - |
dc.contributor.author | Lim, H.M. | - |
dc.contributor.author | Yu, S.-H. | - |
dc.contributor.author | Lee, C.M. | - |
dc.contributor.author | Nam, M.J. | - |
dc.contributor.author | Yang, Y.-H. | - |
dc.contributor.author | Park, K. | - |
dc.contributor.author | Choi, Y.S. | - |
dc.contributor.author | Jang, K.Y. | - |
dc.contributor.author | Park, S.-H. | - |
dc.date.available | 2021-04-09T01:40:12Z | - |
dc.date.created | 2021-01-05 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1520-4081 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80686 | - |
dc.description.abstract | 6,8-Diprenylorobol is a natural compound mainly found in Glycyrrhiza uralensis fisch and Maclura tricuspidata, which has been used traditionally as food and medicine in Asia. So far, the antiproliferative effect of 6,8-diprenylorobol has not been studied yet in colon cancer. In this study, we aimed to evaluate the antiproliferative effects of 6,8-diprenylorobol in LoVo and HCT15, two kinds of human colon cancer cells. 6,8-Diprenylorobol inhibited the proliferation of LoVo and HCT15 cells in a dose- and time-dependent manner. A 40 μM of 6,8-diprenylorobol for 72 h reduced both of cell viability under 50%. After treatment of 6,8-diprenylorobol (40 and 60 μM) for 72 h, late apoptotic cell portion in LoVo and HCT15 cells were 24, 70% and 13, 90%, respectively, which was confirmed by checking DNA fragmentation in both cells. Mechanistically, 6,8-diprenylorobol activated p53 and its phosphorylated form (Ser15, Ser20, and Ser46) expression but suppressed Akt and mitogen-activated protein kinases (MAPKs) phosphorylation in LoVo and HCT15 cells. Interestingly, 6,8-diprenylorobol induced the generation of intracellular reactive oxygen species (ROS), which was attenuated with N-acetyl cysteine (NAC) treatment. Compared to the control, 60 μM of 6,8-diprenylorobol caused to increase ROS level to 210% in LoVo and HCT15, which was reduced into 161% and 124%, respectively with NAC. Furthermore, cell viability and apoptotic cell portion by 6,8-diprenylorobol was recovered by incubation with NAC. Taken together, these results indicate that 6,8-diprenylorobol has the potential antiproliferative effect against LoVo and HCT15 colon cancer cells through activation of p53 and generation of ROS. © 2020 Wiley Periodicals LLC. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | ENVIRONMENTAL TOXICOLOGY | - |
dc.title | 6,8-Diprenylorobol induces apoptosis in human colon cancer cells via activation of intracellular reactive oxygen species and p53 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000603697200001 | - |
dc.identifier.doi | 10.1002/tox.23093 | - |
dc.identifier.bibliographicCitation | ENVIRONMENTAL TOXICOLOGY, v.36, no.5, pp.914 - 925 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85098443973 | - |
dc.citation.endPage | 925 | - |
dc.citation.startPage | 914 | - |
dc.citation.title | ENVIRONMENTAL TOXICOLOGY | - |
dc.citation.volume | 36 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Lee, H.K. | - |
dc.contributor.affiliatedAuthor | Lim, H.M. | - |
dc.contributor.affiliatedAuthor | Nam, M.J. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | 6,8-diprenylorobol | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | colon cancer | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordPlus | Amino acids | - |
dc.subject.keywordPlus | Cell death | - |
dc.subject.keywordPlus | Chemical activation | - |
dc.subject.keywordPlus | Oxygen | - |
dc.subject.keywordPlus | Phosphorylation | - |
dc.subject.keywordPlus | Reactive oxygen species | - |
dc.subject.keywordPlus | Antiproliferative effect | - |
dc.subject.keywordPlus | Colon cancer cells | - |
dc.subject.keywordPlus | DNA fragmentation | - |
dc.subject.keywordPlus | Glycyrrhiza uralensis Fisch | - |
dc.subject.keywordPlus | Human colon cancer cells | - |
dc.subject.keywordPlus | Mitogen activated protein kinase | - |
dc.subject.keywordPlus | N-acetyl cysteines | - |
dc.subject.keywordPlus | Natural compounds | - |
dc.subject.keywordPlus | Diseases | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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