In Vitro Studies to Assess the alpha-Glucosidase Inhibitory Activity and Insulin Secretion Effect of Isorhamnetin 3-O-Glucoside and Quercetin 3-O-Glucoside Isolated from Salicornia herbacea
DC Field | Value | Language |
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dc.contributor.author | Lee, Dahae | - |
dc.contributor.author | Park, Jun Yeon | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.date.available | 2021-04-13T00:40:11Z | - |
dc.date.created | 2021-04-01 | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 2227-9717 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80720 | - |
dc.description.abstract | In this study, we examined the effect of ethanolic extract of Salicornia herbacea (ESH), isorhamnetin 3-O-glucoside (I3G), quercetin 3-O-glucoside (Q3G), quercetin, and isorhamnetin on α-glucosidase activity and glucose-stimulated insulin secretion (GSIS) in insulin-secreting rat insulinoma (INS-1) cells. A portion of the ethyl acetate fraction of ESH was chromatographed on a silica gel by a gradient elution with chloroform and methanol to provide Q3G and I3G. ESH, Q3G, and quercetin inhibited α-glucosidase activity, and quercetin (IC50 value was 29.47 ± 3.36 µM) inhibited the activity more effectively than Q3G. We further demonstrated that ESH, Q3G, quercetin, I3G, and isorhamnetin promote GSIS in INS-1 pancreatic β-cells without inducing cytotoxicity. Among them, I3G was the most effective in enhancing GSIS. I3G enhanced the phosphorylation of total extracellular signal-regulated kinase (ERK), insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), Akt, and activated pancreatic and duodenal homeobox-1 (PDX-1), which are associated with insulin secretion and β-cell function. As components of ESH, Q3G has the potential to regulate blood glucose by inhibiting α-glucosidase activity, and I3G enhances the insulin secretion, but its bioavailability should be considered in determining biological importance. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | PROCESSES | - |
dc.title | In Vitro Studies to Assess the alpha-Glucosidase Inhibitory Activity and Insulin Secretion Effect of Isorhamnetin 3-O-Glucoside and Quercetin 3-O-Glucoside Isolated from Salicornia herbacea | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000634229600001 | - |
dc.identifier.doi | 10.3390/pr9030483 | - |
dc.identifier.bibliographicCitation | PROCESSES, v.9, no.3 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85102986672 | - |
dc.citation.title | PROCESSES | - |
dc.citation.volume | 9 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Lee, Dahae | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | AKT | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | Insulin | - |
dc.subject.keywordAuthor | PDX-1 | - |
dc.subject.keywordAuthor | PI3K | - |
dc.subject.keywordAuthor | Salicornia herbacea | - |
dc.subject.keywordAuthor | α-glucosidase | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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