An acetylcholinesterase-based biosensor for the detection of pesticides using liquid crystals confined in microcapillaries
DC Field | Value | Language |
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dc.contributor.author | Nguyen, Duy Khiem | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2021-04-19T00:40:55Z | - |
dc.date.created | 2021-02-04 | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80774 | - |
dc.description.abstract | Here, we demonstrate a capillary-sensing platform based on liquid crystals (LCs) confined in microcapillaries for simple and sensitive detection of acetylcholinesterase (AChE) and its inhibitors. LC droplets were formed through sequential injection of LCs and an aqueous solution into trichloro(octyl)silane (OTS)-treated microcapillaries. When the confined LC droplets make contact with a cationic surfactant solution, myristoylcholine chloride (Myr), the formation of a Myr monolayer at the aqueous/LC interface induces a horizontal orientation of the LCs at the interface along the microcapillary, producing an optical LC droplet texture of a four-petal shape. On the other hand, AChE can catalyze the hydrolysis of Myr into choline and myristic acid. The hydrolyzed Myr is unable to form a monolayer at the aqueous/LC interface, and therefore the confined LC droplets exhibit two bright-lined optical images when in contact with the pre-incubated mixture of Myr and AChE, corresponding to the homeotropic orientation of LCs at the interface. However, in the presence of AChE-inhibiting pesticides, such as fenobucarb and malathion, the activity of AChE is inhibited, and thus, the enzymatic hydrolysis of Myr cannot occur. As a result, the confined LC droplets present the four petal-shaped optical images when in contact with the pre-incubated mixture of Myr, AChE, and pesticides. Based on this principle, an LC-based microcapillary sensor was developed and utilized for the detection of pesticides. Using this sensing platform, fenobucarb and malathion were detected at limits of 5 pg/mL and 2.5 pg/mL, respectively. Moreover, the proposed biosensor was successfully applied to the determination of pesticides in real river water. Therefore, this LC-based microcapillary sensor is a promising platform for simple, rapid, and label-free detection of pesticides with very high sensitivity. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.title | An acetylcholinesterase-based biosensor for the detection of pesticides using liquid crystals confined in microcapillaries | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000634559200005 | - |
dc.identifier.doi | 10.1016/j.colsurfb.2021.111587 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.200 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85099949386 | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 200 | - |
dc.contributor.affiliatedAuthor | Nguyen, Duy Khiem | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Acetylcholinesterase | - |
dc.subject.keywordAuthor | Inhibitors | - |
dc.subject.keywordAuthor | Liquid crystals | - |
dc.subject.keywordAuthor | Microcapillary biosensor | - |
dc.subject.keywordAuthor | Myristoylcholine | - |
dc.subject.keywordAuthor | Pesticides | - |
dc.subject.keywordPlus | Biosensors | - |
dc.subject.keywordPlus | Cationic surfactants | - |
dc.subject.keywordPlus | Chlorine compounds | - |
dc.subject.keywordPlus | Drops | - |
dc.subject.keywordPlus | Geometrical optics | - |
dc.subject.keywordPlus | Liquid crystals | - |
dc.subject.keywordPlus | Mixtures | - |
dc.subject.keywordPlus | Monolayers | - |
dc.subject.keywordPlus | Pesticides | - |
dc.subject.keywordPlus | Textures | - |
dc.subject.keywordPlus | Acetylcholinesterase | - |
dc.subject.keywordPlus | Cationic surfactant solution | - |
dc.subject.keywordPlus | Homeotropic orientation | - |
dc.subject.keywordPlus | Label-free detection | - |
dc.subject.keywordPlus | Liquid crystals (LCs) | - |
dc.subject.keywordPlus | Micro-capillaries | - |
dc.subject.keywordPlus | Sensitive detection | - |
dc.subject.keywordPlus | Sequential injection | - |
dc.subject.keywordPlus | Enzymatic hydrolysis | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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