Unfolded Protein Corona Surrounding Nanotubes Influence the Innate and Adaptive Immune System
- Authors
- Park, Jun-Young; Park, Sung Jean; Park, Jun Young; Kim, Sang-Hyun; Kwon, Song; Jung, YunJae; Khang, Dongwoo
- Issue Date
- Apr-2021
- Publisher
- WILEY
- Keywords
- conformational changes; immune response; nanotubes; protein corona
- Citation
- Advanced Science, v.8, no.8
- Journal Title
- Advanced Science
- Volume
- 8
- Number
- 8
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80908
- DOI
- 10.1002/advs.202004979
- ISSN
- 2198-3844
- Abstract
- The plasma proteins around nanoparticles (NPs) form an outer protein corona, significantly influencing the subsequent immune response. However, it was uncertain whether the protein corona around NPs influences immune response. This study clarified that the immune response mediated by the protein corona is greatly dependent on the type of plasma proteins surrounding the NPs. Structural changes in the unfolded protein corona elevated reactive oxygen species (ROS) levels and induced major proinflammatory cytokine release in both murine and human macrophage cell lines. In contrast, negligible structural changes in the protein corona provoke neither ROS production nor proinflammatory cytokine release. Furthermore, in vivo analysis confirms that a stimulated immune response by an unfolded protein corona triggers selective activation of innate and adaptive immunity in the spleen. Specifically, neutrophils, natural killer cells, and CD8+ T cells are overpopulated by unfolded protein corona structures surrounding nanotubes, whereas innate and adaptive immunologic responses are not triggered by a normal protein corona. In conclusion, highly unfolded protein corona structures are strongly correlated with subsequent activation of proinflammatory cytokines and innate immune responses; thus, the protein corona can be used in immune-enhancing therapy. © 2021 The Authors. Advanced Science published by Wiley-VCH GmbH
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
- 약학대학 > 약학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80908)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.