A Label-Free Liquid Crystal Biosensor Based on Specific DNA Aptamer Probes for Sensitive Detection of Amoxicillin Antibiotic
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Duy Khiem Nguyen | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.accessioned | 2021-05-10T07:40:16Z | - |
dc.date.available | 2021-05-10T07:40:16Z | - |
dc.date.created | 2021-04-26 | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 2072-666X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80969 | - |
dc.description.abstract | We developed a liquid crystal (LC) aptamer biosensor for the sensitive detection of amoxi-cillin (AMX). The AMX aptamer was immobilized onto the surface of a glass slide modified with a mixed self-assembled layer of dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) and (3-aminopropyl) triethoxysilane (APTES). The long alkyl chains of DMOAP main-tained the LC molecules in a homeotropic orientation and induced a dark optical appearance under a polarized light microscope (POM). In the presence of AMX, the specific binding of the aptamer and AMX molecules induced a conformational change in the aptamers, leading to the disruption of the homeotropic orientation of LCs, resulting in a bright optical appearance. The developed aptasensor showed high specificity and a low detection limit of 3.5 nM. Moreover, the potential application of the developed aptasensor for the detection of AMX in environmental samples was also demonstrated. Therefore, the proposed aptasensor is a promising platform for simple, rapid, and label-free monitoring of AMX in an actual water environment with high selectivity and sensitivity. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Micromachines | - |
dc.title | A Label-Free Liquid Crystal Biosensor Based on Specific DNA Aptamer Probes for Sensitive Detection of Amoxicillin Antibiotic | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000643297700001 | - |
dc.identifier.doi | 10.3390/mi12040370 | - |
dc.identifier.bibliographicCitation | Micromachines, v.12, no.4 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85104309045 | - |
dc.citation.title | Micromachines | - |
dc.citation.volume | 12 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Duy Khiem Nguyen | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Amoxicillin | - |
dc.subject.keywordAuthor | DNA aptamer | - |
dc.subject.keywordAuthor | Liquid crystal biosensors | - |
dc.subject.keywordAuthor | Liquid crystals | - |
dc.subject.keywordAuthor | Polarized light microscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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