Induction of apoptosis in indole-3-carbinol-treated lung cancer H1299 cells via ROS level elevation
DC Field | Value | Language |
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dc.contributor.author | Lim, Heui Min | - |
dc.contributor.author | Park, See-Hyoung | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.date.accessioned | 2021-05-13T01:40:08Z | - |
dc.date.available | 2021-05-13T01:40:08Z | - |
dc.date.created | 2020-11-06 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 0960-3271 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80994 | - |
dc.description.abstract | This study was focused on investigating the anticancer potential of indole-3-carbinol (I3C) against lung cancer H1299 cells via an increase in ROS levels. To investigate the induction of growth arrest and/or cell death in H1299 cells, a cell cycle arrest assay, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) assay, and reactive oxygen species (ROS) detection assay were performed. Through the TUNEL assay, we detected I3C-induced DNA fragmentation. Fluorescence-activated cell sorting (FACS) analysis showed that I3C induced an increase in ROS levels and apoptotic rate in a dose- and time-dependent manner in H1299 cells. Western blotting demonstrated that activated forms of caspase-3, caspase-7, caspase-9, and poly (ADP-ribose) polymerase (PARP) were increased in I3C-treated H1299 cells following treatment with I3C. Furthermore, protein expression levels of FOXO3, bim, bax, and phosphorylated ERK and JNK were increased, while those of pAkt, Bcl-xL, and Bcl-2 were decreased by I3C treatment of H1299 cells. To confirm the relationship between cell apoptosis and ROS generation, H1299 cells were treated with I3C simultaneously with N-acetylcysteine (NAC), and it was shown that ROS levels decreased and viability increased. Moreover, in western blot analysis, expression of anti-apoptotic proteins (thioredoxin1, peroxiredoxin-1, Bcl-2, and Bcl-xL) in I3C-treated cells was evidently downregulated and pro-apoptotic proteins (active ASK1 and cleaved PARP) were upregulated compared to cells co-treated with NAC. The study showed that I3C induced downregulation of ROS regulator proteins and elevation of ROS, thus activating apoptotic signaling cascades in human lung cancer H1299 cells. © The Author(s) 2020. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE Publications Ltd | - |
dc.relation.isPartOf | Human and Experimental Toxicology | - |
dc.title | Induction of apoptosis in indole-3-carbinol-treated lung cancer H1299 cells via ROS level elevation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000636025800001 | - |
dc.identifier.doi | 10.1177/0960327120969968 | - |
dc.identifier.bibliographicCitation | Human and Experimental Toxicology, v.40, no.5, pp.812 - 825 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85094645151 | - |
dc.citation.endPage | 825 | - |
dc.citation.startPage | 812 | - |
dc.citation.title | Human and Experimental Toxicology | - |
dc.citation.volume | 40 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Lim, Heui Min | - |
dc.contributor.affiliatedAuthor | Nam, Myeong Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | H1299 | - |
dc.subject.keywordAuthor | I3C | - |
dc.subject.keywordAuthor | lung cancer | - |
dc.subject.keywordAuthor | reactive oxygen species (ROS) | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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