Proteomic Analysis of the Vitreous Body in Proliferative and Non-Proliferative Diabetic Retinopathy
DC Field | Value | Language |
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dc.contributor.author | DUONG VAN AN | - |
dc.contributor.author | Ahn, Jeeyun | - |
dc.contributor.author | Han, Na-Young | - |
dc.contributor.author | Park, Jong-Moon | - |
dc.contributor.author | Mok, Jeong-Hun | - |
dc.contributor.author | Kim, Tae Wan | - |
dc.contributor.author | Lee, Hookeun | - |
dc.date.accessioned | 2021-06-01T00:40:06Z | - |
dc.date.available | 2021-06-01T00:40:06Z | - |
dc.date.created | 2021-03-03 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1570-1646 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81157 | - |
dc.description.abstract | Background: Diabetic Retinopathy (DR), one of the major microvascular complications commonly occurring in diabetic patients, can be classified into Proliferative Diabetic Retinopathy (PDR) and Non-Proliferative Diabetic Retinopathy (NPDR). Currently, available therapies are only targeted for later stages of the disease in which some pathologic changes may be irreversible. Thus, there is a need to develop new treatment options for earlier stages of DR through revealing patho-logical mechanisms of PDR and NPDR. Objective: The purpose of this study was to characterize the proteomes of diabetes through quantitative analysis of PDR and NPDR. Methods: Vitreous body was collected from three groups: control (non-diabetes mellitus), NPDR, and PDR. Vitreous proteins were digested to peptide mixtures and analyzed using LC-MS/MS. MaxQuant was used to search against the database and statistical analyses were performed using Perseus. Gene ontology analysis, related-disease identification, and protein-protein interaction were performed using the differential expressed proteins. Results: Twenty proteins were identified as critical in PDR and NPDR. The NPDR group showed different expressions of kininogen-1, serotransferrin, ribonuclease pancreatic, osteopontin, keratin type II cytoskeletal 2 epidermal, and transthyretin. Also, prothrombin, signal transducer and activa-tor of transcription 4, hemoglobin subunit alpha, beta, and delta were particularly up-regulated proteins for PDR group. The up-regulated proteins related to complement and coagulate cascades. Statherin was down-regulated in PDR and NPDR compared with the control group. Transthyretin was the unique protein that increased its abundance in NPDR compared with the PDR and control group. Conclusion: This study confirmed the different expressions of some proteins in PDR and NPDR. Additionally, we revealed uniquely expressed proteins of PDR and NPDR, which would be differential biomarkers: prothrombin, alpha-2-HS-glycoprotein, hemoglobin subunit alpha, beta, and tran-sthyretin. © 2021 Bentham Science Publishers. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BENTHAM SCIENCE PUBL LTD | - |
dc.relation.isPartOf | CURRENT PROTEOMICS | - |
dc.title | Proteomic Analysis of the Vitreous Body in Proliferative and Non-Proliferative Diabetic Retinopathy | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000652350800005 | - |
dc.identifier.doi | 10.2174/1570164617666200302101442 | - |
dc.identifier.bibliographicCitation | CURRENT PROTEOMICS, v.18, no.2, pp.143 - 152 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85101235673 | - |
dc.citation.endPage | 152 | - |
dc.citation.startPage | 143 | - |
dc.citation.title | CURRENT PROTEOMICS | - |
dc.citation.volume | 18 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | DUONG VAN AN | - |
dc.contributor.affiliatedAuthor | Han, Na-Young | - |
dc.contributor.affiliatedAuthor | Park, Jong-Moon | - |
dc.contributor.affiliatedAuthor | Mok, Jeong-Hun | - |
dc.contributor.affiliatedAuthor | Lee, Hookeun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Gene ontology | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | Non-proliferative diabetic retinopathy | - |
dc.subject.keywordAuthor | Proliferative diabetic retinopathy | - |
dc.subject.keywordAuthor | Proteomics | - |
dc.subject.keywordAuthor | Vitreous body | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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