Attenuation of Inflammatory Symptoms by Icariside B2 in Carrageenan and LPS-Induced Inflammation Models via Regulation of MAPK/NF-kappa B Signaling Cascades
DC Field | Value | Language |
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dc.contributor.author | Alam, Md Badrul | - |
dc.contributor.author | Kwon, Yoon-Gyung | - |
dc.contributor.author | Simu, Shakina Yesmin | - |
dc.contributor.author | Shahriyar, Sk Abrar | - |
dc.contributor.author | Lee, Sang Han | - |
dc.date.accessioned | 2021-06-11T06:40:24Z | - |
dc.date.available | 2021-06-11T06:40:24Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 2218-273X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81254 | - |
dc.description.abstract | Prolonged inflammatory responses can lead to the development of several chronic diseases, such as autoimmune disorders and the development of natural therapeutic agents is required. A murine model was used to assess the anti-inflammatory effects of the megastigmane glucoside, icariside B2 (ICSB), and the assessment was carried out in vitro, and in vivo. The in vitro anti-inflammatory effects of ICSB were tested using LPS-stimulated BV2 cells, and the protein expression levels of inflammatory genes and cytokines were assessed. Mice were subcutaneously injected with 1% carrageenan (CA) to induce acute phase inflammation in the paw. Inflammation was assessed by measuring paw volumes hourly; subsequently, the mice were euthanized and the right hind paw skin was expunged and processed for reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses. ICSB inhibits LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE(2)) generation by reducing the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). ICSB also inhibits the COX-2 enzyme with an IC50 value of 7.80 +/- 0.26 mu M. Molecular docking analysis revealed that ICSB had a strong binding affinity with both murine and human COX-2 proteins with binding energies of -8 kcal/mol and -7.4 kcal/mol, respectively. ICSB also reduces the manifestation of pro-inflammatory cytokines, such as TNF-alpha, IL-6, and IL-1 beta, at their transcriptional and translational level. ICSB hinders inhibitory protein kappa B alpha (I kappa B alpha) phosphorylation, thereby terminating the nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kappa B) nuclear translocation. ICSB also represses the mitogen-activated protein kinases (MAPKs) signaling pathways. ICSB (50 mg/kg) showed an anti-edema effect in CA-induced mice and suppressed the CA-induced increases in iNOS and COX-2 protein levels. ICSB attenuated inflammatory responses by downregulating NF-kappa B expression through interference with extracellular signal-regulated kinase (ERK) and p38 phosphorylation, and by modulating the expression levels of iNOS, COX-2, TNF-alpha, IL-1 beta, and IL-6. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | BIOMOLECULES | - |
dc.title | Attenuation of Inflammatory Symptoms by Icariside B2 in Carrageenan and LPS-Induced Inflammation Models via Regulation of MAPK/NF-kappa B Signaling Cascades | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000556994900001 | - |
dc.identifier.doi | 10.3390/biom10071037 | - |
dc.identifier.bibliographicCitation | BIOMOLECULES, v.10, no.7 | - |
dc.description.isOpenAccess | N | - |
dc.citation.title | BIOMOLECULES | - |
dc.citation.volume | 10 | - |
dc.citation.number | 7 | - |
dc.contributor.affiliatedAuthor | Simu, Shakina Yesmin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | anti-inflammatory effects | - |
dc.subject.keywordAuthor | icariside B2 | - |
dc.subject.keywordAuthor | NF-kappa B signaling | - |
dc.subject.keywordAuthor | MAP-kinase | - |
dc.subject.keywordPlus | ANTIINFLAMMATORY ACTIVITY | - |
dc.subject.keywordPlus | INDUCED EDEMA | - |
dc.subject.keywordPlus | LUNG INJURY | - |
dc.subject.keywordPlus | LIGNAN | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | PAW | - |
dc.subject.keywordPlus | L. | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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