Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kumar, Shiv | - |
dc.contributor.author | Umair, Zobia | - |
dc.contributor.author | Kumar, Vijay | - |
dc.contributor.author | Lee, Unjoo | - |
dc.contributor.author | Choi, Sun-Cheol | - |
dc.contributor.author | Kim, Jaebong | - |
dc.date.accessioned | 2021-06-16T02:41:57Z | - |
dc.date.available | 2021-06-16T02:41:57Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81319 | - |
dc.description.abstract | Dorsoventral patterning of body axis in vertebrate embryo is tightly controlled by a complex regulatory network of transcription factors. Ventx1.1 is known as a transcriptional repressor to inhibit dorsal mesoderm formation and neural differentiation in Xenopus. In an attempt to identify, using chromatin immunoprecipitation (ChIP)-Seq, genome-wide binding pattern of Ventx1.1 in Xenopus gastrulae, we observed that Ventx1.1 associates with its own 5'-flanking sequence. In this study, we present evidence that Ventx1.1 binds a cis-acting Ventx1.1 response element (VRE) in its own promoter, leading to repression of its own transcription. Site-directed mutagenesis of the VRE in the Ventx1.1 promoter significantly abrogated this inhibitory autoregulation of Ventx1.1 transcription. Notably, Ventx1.1 and Xcad2, an activator of Ventx1.1 transcription, competitively co-occupied the VRE in the Ventx1.1 promoter. In support of this, mutation of the VRE down-regulated basal and Xcad2-induced levels of Ventx1.1 promoter activity. In addition, overexpression of Ventx1.1 prevented Xcad2 from binding to the Ventx1.1 promoter, and vice versa. Taken together, these results suggest that Ventx1.1 negatively regulates its own transcription in competition with Xcad2, thereby fine-tuning its own expression levels during dorsoventral patterning of Xenopus early embryo. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.title | Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000473211900008 | - |
dc.identifier.doi | 10.5483/BMBRep.2019.52.6.085 | - |
dc.identifier.bibliographicCitation | BMB REPORTS, v.52, no.6, pp.403 - 408 | - |
dc.identifier.kciid | ART002475759 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85068961571 | - |
dc.citation.endPage | 408 | - |
dc.citation.startPage | 403 | - |
dc.citation.title | BMB REPORTS | - |
dc.citation.volume | 52 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Umair, Zobia | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Autoregulation | - |
dc.subject.keywordAuthor | Transcription | - |
dc.subject.keywordAuthor | Ventx1.1 | - |
dc.subject.keywordAuthor | Xcad2 | - |
dc.subject.keywordAuthor | Xenopus | - |
dc.subject.keywordPlus | HOMEOBOX GENE | - |
dc.subject.keywordPlus | XVENT-2 | - |
dc.subject.keywordPlus | AXIS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.