Highly specific SNP detection using 2D graphene electronics and DNA strand displacement
DC Field | Value | Language |
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dc.contributor.author | Hwang, Michael T. | - |
dc.contributor.author | Landon, Preston B. | - |
dc.contributor.author | Lee, Joon | - |
dc.contributor.author | Choi, Duyoung | - |
dc.contributor.author | Mo, Alexander H. | - |
dc.contributor.author | Glinsky, Gennadi | - |
dc.contributor.author | Lal, Ratnesh | - |
dc.date.accessioned | 2021-06-17T00:40:19Z | - |
dc.date.available | 2021-06-17T00:40:19Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81335 | - |
dc.description.abstract | Single-nucleotide polymorphisms (SNPs) in a gene sequence are markers for a variety of human diseases. Detection of SNPs with high specificity and sensitivity is essential for effective practical implementation of personalized medicine. Current DNA sequencing, including SNP detection, primarily uses enzyme-based methods or fluorophore-labeled assays that are time-consuming, need laboratory-scale settings, and are expensive. Previously reported electrical charge-based SNP detectors have insufficient specificity and accuracy, limiting their effectiveness. Here, we demonstrate the use of a DNA strand displacement-based probe on a graphene field effect transistor (FET) for high-specificity, single-nucleotide mismatch detection. The single mismatch was detected by measuring strand displacement-induced resistance (and hence current) change and Dirac point shift in a graphene FET. SNP detection in large double-helix DNA strands (e.g., 47 nt) minimize false-positive results. Our electrical sensor-based SNP detection technology, without labeling and without apparent cross-hybridization artifacts, would allow fast, sensitive, and portable SNP detection with single-nucleotide resolution. The technology will have a wide range of applications in digital and implantable biosensors and high-throughput DNA genotyping, with transformative implications for personalized medicine. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.title | Highly specific SNP detection using 2D graphene electronics and DNA strand displacement | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000379033400046 | - |
dc.identifier.doi | 10.1073/pnas.1603753113 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.113, no.26, pp.7088 - 7093 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 7093 | - |
dc.citation.startPage | 7088 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 113 | - |
dc.citation.number | 26 | - |
dc.contributor.affiliatedAuthor | Hwang, Michael T. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | bioelectronics | - |
dc.subject.keywordAuthor | graphene FET DNA sensor | - |
dc.subject.keywordAuthor | electrical biosensor | - |
dc.subject.keywordAuthor | DNA strand displacement | - |
dc.subject.keywordAuthor | SNP detection | - |
dc.subject.keywordPlus | FIELD-EFFECT TRANSISTOR | - |
dc.subject.keywordPlus | CVD-GROWN GRAPHENE | - |
dc.subject.keywordPlus | LABEL-FREE DETECTION | - |
dc.subject.keywordPlus | SILICON-NANOWIRE | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | SINGLE | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | BIOMOLECULES | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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