Purification and characterization of chitinase showing antifungal and biodegradation properties obtained from Streptomyces anulatus CS242
- Authors
- Mander, Poonam; Cho, Seung Sik; Choi, Yun Hee; Panthi, Sandesh; Choi, Yoon Seok; Kim, Hwan Mook; Yoo, Jin Cheol
- Issue Date
- Jul-2016
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Antifungal enzyme; Chitinase; Chitinous shrimp waste; Chitooligosaccharide; Streptomyces anulatus
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.39, no.7, pp.878 - 886
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 39
- Number
- 7
- Start Page
- 878
- End Page
- 886
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8147
- DOI
- 10.1007/s12272-016-0747-3
- ISSN
- 0253-6269
- Abstract
- In an effort to identify a microbial enzyme that can be useful as a fungicide and biodegradation agent of chitinous wastes, a chitinase (Chi242) was purified from the culture supernatant of Streptomyces anulatus CS242 utilizing powder of shrimp shell wastes as a sole carbon source. It was purified employing ammonium sulfate precipitation and gel permeation chromatography techniques. The molecular weight of the purified chitinase was similar to 38 kDa by SDS-PAGE. The N-terminal amino acid sequence (A-P-G-A-P-G-T-G-A-L) showed close similarity to those of other Streptomyes chitinases. The purified enzyme displayed optimal activity at pH 6.0 and 50 A degrees C respectively. It showed substantial thermal stability for 2 h at 30-60 A degrees C, and exhibited broad pH stability in the range 5.0-13.0 for 48 h at 4 A degrees C. Scanning electron microscopy confirmed the ability of this enzyme to adsorb onto solid shrimp bio-waste and to degrade chitin microfibers. Chi242 could proficiently convert colloidal chitin to N-acetyl glucosamine (GlcNAc) and N-acetyl chitobiose (GlcNAc)2 signifying that this enzyme is suitable for bioconversion of chitin waste. In addition, it exerted an effective antifungal activity towards fungal pathogen signifying its role as a biocontrol agent. Thus, a single microbial cell of Streptomyces anulatus CS242 justified its dual role.
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