Effect of mesenchymal stromal cells encapsulated within polyethylene glycol-collagen hydrogels formed in situ on alkali-burned corneas in an ex vivo organ culture model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Na, Kyung-Sun | - |
dc.contributor.author | Fernandes-Cunha, Gabriella Maria | - |
dc.contributor.author | Varela, Ignacio Blanco | - |
dc.contributor.author | Lee, Hyun Jong | - |
dc.contributor.author | Seo, Youngyoon Amy | - |
dc.contributor.author | Myung, David | - |
dc.date.accessioned | 2021-07-04T05:41:44Z | - |
dc.date.available | 2021-07-04T05:41:44Z | - |
dc.date.created | 2021-04-01 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.issn | 1465-3249 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81531 | - |
dc.description.abstract | Background aims: Corneal inflammation after alkali burns often results in vision loss due to corneal opacification and neovascularization. Mesenchymal stem cells (MSCs) and their secreted factors (secretome) have been studied for their anti-inflammatory and anti-angiogenic properties with encouraging results. However, topical instillation of MSCs or their secretome is often accompanied by issues related to delivery or rapid washout. Polyethylene glycol (PEG) and collagen are well-known biomaterials used extensively in scaffolds for tissue engineering. To effectively suppress alkaline burn-induced corneal injury, the authors proposed encapsulating MSCs within collagen gels cross-linked with multi-functional PEG-succinimidyl esters as a means to deliver the secretome of immobilized MSCs. Methods: Human MSCs were added to a neutralized collagen solution and mixed with a solution of four-arm PEG-N-hydroxysuccinimide. An ex vivo organ culture was conducted using rabbit corneas injured by alkali burn. MSCs were encapsulated within PEG-collagen hydrogels and injected onto the wounded cornea immediately following alkali burn and washing. Photographs of the ocular surface were taken over a period of 7 days after the alkali burn and processed for immunohistochemical evaluation. Samples were split into three groups: injury without treatment, MSCs alone, and MSCs encapsulated within PEG-collagen hydrogels. Results: All corneas in ex vivo organ culture lost their transparency immediately after alkali burn, and only the groups treated with MSCs and MSCs encapsulated within PEG-collagen hydrogels recovered some transparency after 7 days. Immunohistochemical analysis revealed increased expression of vimentin in the anterior corneal stroma of the group without treatment indicative of fibrotic healing, whereas less stromal vimentin was detected in the group containing MSCs encapsulated within the PEG-collagen hydrogels. Conclusions: PEG-collagen hydrogels enable the encapsulation of viable MSCs capable of releasing secreted factors onto the ocular surface. Encapsulating MSCs within PEG-collagen hydrogels may be a promising method for delivering their therapeutic benefits in cases of ocular inflammatory diseases, such as alkali burn injuries. © 2021 | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | CYTOTHERAPY | - |
dc.title | Effect of mesenchymal stromal cells encapsulated within polyethylene glycol-collagen hydrogels formed in situ on alkali-burned corneas in an ex vivo organ culture model | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000651257600005 | - |
dc.identifier.doi | 10.1016/j.jcyt.2021.02.001 | - |
dc.identifier.bibliographicCitation | CYTOTHERAPY, v.23, no.6, pp.500 - 509 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85103043332 | - |
dc.citation.endPage | 509 | - |
dc.citation.startPage | 500 | - |
dc.citation.title | CYTOTHERAPY | - |
dc.citation.volume | 23 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Jong | - |
dc.contributor.affiliatedAuthor | Myung, David | - |
dc.type.docType | Article in Press | - |
dc.subject.keywordAuthor | collagen | - |
dc.subject.keywordAuthor | cornea | - |
dc.subject.keywordAuthor | encapsulation | - |
dc.subject.keywordAuthor | mesenchymal stromal cells | - |
dc.subject.keywordAuthor | regeneration | - |
dc.subject.keywordAuthor | wound healing | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.