Microfluidic electrical cell lysis for high-throughput and continuous production of cell-free varicella-zoster virus
DC Field | Value | Language |
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dc.contributor.author | Won, Eun-Jae | - |
dc.contributor.author | Thai, Duc Anh | - |
dc.contributor.author | Duong, Duong Duy | - |
dc.contributor.author | Lee, Nae Yoon | - |
dc.contributor.author | Song, Yoon-Jae | - |
dc.date.accessioned | 2021-07-06T01:40:29Z | - |
dc.date.available | 2021-07-06T01:40:29Z | - |
dc.date.created | 2021-06-25 | - |
dc.date.issued | 2021-07-20 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81599 | - |
dc.description.abstract | Varicella-zoster virus (VZV), the causative agent of varicella and herpes zoster, is highly cell-associated and spreads via cell-to-cell contact in tissue culture. The lack of cell-free VZV hampers studies on VZV biology as well as antiviral and vaccine development. In the present study, a poly(methylmethacrylate) microfluidic device integrated with arrays of microelectrode was fabricated to continuously electrolyse VZV-infected cells to produce cell-free viruses. By designing multiple constrictions and microelectrode arrays, a high electric field is focused on the constricted region of the microchannel to disrupt large numbers of virus-infected cells with high-throughput on a microfluidic platform. Plaque assay and scanning electron microscopy were conducted to quantify and characterize cell-free VZV produced using the microfluidic continuous-flow electrical cell lysis device. The process of microfluidic electrical cell lysis followed by subsequent filtration and virus concentration process yielded a 1.4–2.1 × 104 plaque-forming units (PFUs) per mL of cell-free VZV from 7.0 × 106 VZV-infected human foreskin fibroblasts (HFF) cells. The high electric field formed inside a microfluidic channel combined with the continuous-flow of virus-infected cells within the microchannel enabled the rapid and efficient production of high-titer cell-free virus in large quantities with relatively low input of the voltage. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.relation.isPartOf | Journal of Biotechnology | - |
dc.title | Microfluidic electrical cell lysis for high-throughput and continuous production of cell-free varicella-zoster virus | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000662890900003 | - |
dc.identifier.doi | 10.1016/j.jbiotec.2021.06.006 | - |
dc.identifier.bibliographicCitation | Journal of Biotechnology, v.335, pp.19 - 26 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85108151032 | - |
dc.citation.endPage | 26 | - |
dc.citation.startPage | 19 | - |
dc.citation.title | Journal of Biotechnology | - |
dc.citation.volume | 335 | - |
dc.contributor.affiliatedAuthor | Won, Eun-Jae | - |
dc.contributor.affiliatedAuthor | Thai, Duc Anh | - |
dc.contributor.affiliatedAuthor | Duong, Duong Duy | - |
dc.contributor.affiliatedAuthor | Lee, Nae Yoon | - |
dc.contributor.affiliatedAuthor | Song, Yoon-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Cell-free VZV | - |
dc.subject.keywordAuthor | Electrolysis | - |
dc.subject.keywordAuthor | Focused electric field | - |
dc.subject.keywordAuthor | Microfluidics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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