Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction
DC Field | Value | Language |
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dc.contributor.author | 박다솜 | - |
dc.contributor.author | 박종현 | - |
dc.date.accessioned | 2021-07-22T02:40:52Z | - |
dc.date.available | 2021-07-22T02:40:52Z | - |
dc.date.created | 2021-06-03 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81732 | - |
dc.description.abstract | A risk analysis of Shiga toxin (Stx)-encoding bacteriophage was carried out by confirming the transduction phage to non-Stx-producing Escherichia coli (STEC) and subsequent expression of the Shiga toxin genes. The virulence factor stx1 was identified in five phages, and both stx1 and stx2 were found in four phages from a total of 19 phage isolates with seven non-O157 STEC strains. The four phages, designated as φNOEC41, φNOEC46, φNOEC47, and φNOEC49, belonged morphologically to the Myoviridae family. The stabilities of these phages to temperature, pH, ethanol, and NaClO were high with some variabilities among the phages. The infection of five non-STEC strains by nine Stx-encoding phages occurred at a rate of approximately 40%. Non-STEC strains were transduced by Stx-encoding phage to become lysogenic strains, and seven convertant strains had stx1 and/or stx2 genes. Only the stx1 gene was transferred to the receptor strains without any deletion. Gene expression of a convertant having both stx1 and stx2 genes was confirmed to be up to 32 times higher for Stx1 in 6% NaCl osmotic media and twice for Stx2 in 4% NaCl media, compared with expression in low-salt environments. Therefore, a new risk might arise from the transfer of pathogenic genes from Stx-encoding phages to otherwise harmless hosts. Without adequate sterilization of food exposed to various environments, there is a possibility that the toxicity of the phages might increase. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.relation.isPartOf | Journal of Microbiology and Biotechnology | - |
dc.title | Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction | - |
dc.title.alternative | Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000672695000009 | - |
dc.identifier.doi | 10.4014/jmb.2102.02040 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.31, no.5, pp.710 - 716 | - |
dc.identifier.kciid | ART002721958 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85106977768 | - |
dc.citation.endPage | 716 | - |
dc.citation.startPage | 710 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 31 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | 박다솜 | - |
dc.contributor.affiliatedAuthor | 박종현 | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | Shiga toxin | - |
dc.subject.keywordAuthor | transduction | - |
dc.subject.keywordAuthor | non-pathogenic E. coli | - |
dc.subject.keywordAuthor | convertant | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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