Optimization of adipogenic differentiation conditions for canine adipose-derived stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jong-Yeon | - |
dc.contributor.author | Park, Eun-Jung | - |
dc.contributor.author | Kim, Sung-Min | - |
dc.contributor.author | Lee, Hae-Jeung | - |
dc.date.accessioned | 2021-08-05T01:40:34Z | - |
dc.date.available | 2021-08-05T01:40:34Z | - |
dc.date.created | 2021-07-17 | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81824 | - |
dc.description.abstract | Background: Canine adipose-derived stem cells (cADSCs) exhibit various differentiation properties and are isolated from the canine subcutaneous fat. Although cADSCs are valuable as tools for research on adipogenic differentiation, studies focusing on adipogenic differentiation methods and the underlying mechanisms are still lacking. Objectives: In this study, we aimed to establish an optimal method for adipogenic differentiation conditions of cADSCs and evaluate the role of peroxisome proliferator-activated receptor gamma (PPARγ) and estrogen receptor (ER) signaling in the adipogenic differentiation. Methods: To induce adipogenic differentiation of cADSCs, 3 different adipogenic medium conditions, MDI, DRI, and MDRI, using 3-isobutyl-1-methylxanthine (M), dexamethasone (D), insulin (I), and rosiglitazone (R) were tested. Results: MDRI, addition of PPARγ agonist rosiglitazone to MDI, was the most significantly facilitated cADSC into adipocyte. GW9662, an antagonist of PPARγ, significantly reduced adipogenic differentiation induced by rosiglitazone. Adipogenic differentiation was also stimulated when 17β-estradiol was added to MDI and DRI, and this stimulation was inhibited by the ER antagonist ICI182,780. Conclusions: Taken together, our results suggest that PPARγ and ER signaling are related to the adipogenic differentiation of cADSCs. This study could provide basic information for future research on obesity or anti-obesity mechanisms in dogs. © 2021. The Korean Society of Veterinary Science. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Korean Society of Veterinary Science | - |
dc.relation.isPartOf | Journal of Veterinary Science | - |
dc.title | Optimization of adipogenic differentiation conditions for canine adipose-derived stem cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000677673000009 | - |
dc.identifier.doi | 10.4142/JVS.2021.22.E53 | - |
dc.identifier.bibliographicCitation | Journal of Veterinary Science, v.22, no.4, pp.0 - 0 | - |
dc.identifier.kciid | ART002740404 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85109437349 | - |
dc.citation.endPage | 0 | - |
dc.citation.startPage | 0 | - |
dc.citation.title | Journal of Veterinary Science | - |
dc.citation.volume | 22 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Yeon | - |
dc.contributor.affiliatedAuthor | Park, Eun-Jung | - |
dc.contributor.affiliatedAuthor | Kim, Sung-Min | - |
dc.contributor.affiliatedAuthor | Lee, Hae-Jeung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | adipogenesis | - |
dc.subject.keywordAuthor | canine adipose-derived stem cells | - |
dc.subject.keywordAuthor | Dogs | - |
dc.subject.keywordAuthor | estrogen receptor | - |
dc.subject.keywordAuthor | PPAR gamma | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.