Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing
DC Field | Value | Language |
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dc.contributor.author | Choi, Won Seok | - |
dc.contributor.author | Kim, Joo Hyun | - |
dc.contributor.author | Ahn, Chi Bum | - |
dc.contributor.author | Lee, Ji Hyun | - |
dc.contributor.author | Kim, Yu Jin | - |
dc.contributor.author | Son, Kuk Hui | - |
dc.contributor.author | Lee, Jin Woo | - |
dc.date.accessioned | 2021-09-06T00:41:28Z | - |
dc.date.available | 2021-09-06T00:41:28Z | - |
dc.date.created | 2021-08-23 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82071 | - |
dc.description.abstract | Large-sized or deep skin wounds require skin substitutes for proper healing without scar formation. Therefore, multi-layered skin substitutes that mimic the genuine skin anatomy of multiple layers have attracted attention as suitable skin substitutes. In this study, a novel skin substitute was developed by combining the multi-layer skin tissue reconstruction method with the combination of a human-derived keratinic extract-loaded nano-and micro-fiber using electrospinning and a support structure using 3D printing. A polycaprolactone PCL/keratin electrospun scaffold showed better cell adhesion and proliferation than the keratin-free PCL scaffold, and keratinocytes and fibroblasts showed better survival, adhesion, and proliferation in the PCL/keratin electrospun nanofiber scaffold and microfiber scaffold, respectively. In a co-culture of keratinocytes and fibroblasts using a multi-layered scaffold, the two cells formed the epidermis and dermal layer on the PCL/keratin scaffold without territorial invasion. In the animal study, the PCL/keratin scaffold caused a faster regeneration of new skin without scar formation compared to the PCL scaffold. Our study showed that PCL/keratin scaffolds co-cultured with keratinocytes and fibroblasts promoted the regeneration of the epidermal and dermal layers in deep skin defects. Such finding suggests a new possibility for artificial skin production using multiple cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Polymers | - |
dc.title | Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000689870100001 | - |
dc.identifier.doi | 10.3390/polym13162584 | - |
dc.identifier.bibliographicCitation | Polymers, v.13, no.16 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85112474223 | - |
dc.citation.title | Polymers | - |
dc.citation.volume | 13 | - |
dc.citation.number | 16 | - |
dc.contributor.affiliatedAuthor | Choi, Won Seok | - |
dc.contributor.affiliatedAuthor | Kim, Joo Hyun | - |
dc.contributor.affiliatedAuthor | Ahn, Chi Bum | - |
dc.contributor.affiliatedAuthor | Lee, Ji Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Yu Jin | - |
dc.contributor.affiliatedAuthor | Son, Kuk Hui | - |
dc.contributor.affiliatedAuthor | Lee, Jin Woo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | 3D printing | - |
dc.subject.keywordAuthor | Co-culture | - |
dc.subject.keywordAuthor | Electrospinning | - |
dc.subject.keywordAuthor | Human hair keratinic extract | - |
dc.subject.keywordAuthor | Skin substitute | - |
dc.subject.keywordPlus | Artificial organs | - |
dc.subject.keywordPlus | Cell adhesion | - |
dc.subject.keywordPlus | Cell culture | - |
dc.subject.keywordPlus | Fibroblasts | - |
dc.subject.keywordPlus | Scaffolds (biology) | - |
dc.subject.keywordPlus | Tissue regeneration | - |
dc.subject.keywordPlus | Animal studies | - |
dc.subject.keywordPlus | Artificial skin | - |
dc.subject.keywordPlus | Electrospun nanofibers | - |
dc.subject.keywordPlus | Electrospun scaffolds | - |
dc.subject.keywordPlus | Multiple layers | - |
dc.subject.keywordPlus | Scar formation | - |
dc.subject.keywordPlus | Skin substitutes | - |
dc.subject.keywordPlus | Support structures | - |
dc.subject.keywordPlus | 3D printers | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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