Methylglyoxal-derived hemoglobin advanced glycation end products induce apoptosis and oxidative stress in human umbilical vein endothelial cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae Hyuk | - |
dc.contributor.author | Samsuzzaman, M. | - |
dc.contributor.author | Park, Myoung Gyu | - |
dc.contributor.author | Park, Sung Jean | - |
dc.contributor.author | Kim, Sun Yeou | - |
dc.date.accessioned | 2021-09-09T00:40:30Z | - |
dc.date.available | 2021-09-09T00:40:30Z | - |
dc.date.created | 2021-08-05 | - |
dc.date.issued | 2021-09-30 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82089 | - |
dc.description.abstract | The presence of excess glucose promotes hemoglobin glycation via the biochemical modification of hemoglobin by dicarbonyl products. However, the precise effects of Hb-AGEs in human umbilical vein endothelial cells (HUVECs) are not known to date. Therefore, we investigated the tentative effects of Hb-AGEs in HUVECs. Initially, we used the AGE formation assay to examine the selectivity of MGO toward various proteins. Among all proteins, MGO-Hb-AGEs formation was higher compared to the formation of other dicarbonyl-mediated AGEs. Our next data demonstrated that treatment with 0.5 mg/mL of Hb-AGEs-4w significantly reduced cell viability in HUVECs. Further, we evaluated the role of MGO in conformational and structural changes in Hb. The results showed that Hb demonstrated a highly altered conformation upon incubation with MGO. Moreover, Hb-AGEs-4w treatment strongly increased ROS production, and decreased mitochondrial membrane potential in HUVECs, and moderately reduced the expression of phosphorylated forms of p-38 and JNK. We observed that Hb-AGEs-4w treatment increased the number of apoptotic cells and the Bax/Bcl-2 ratio and cleaved the nuclear enzyme PARP in HUVECs. Finally, Hb-AGEs also inhibited migration and proliferation of HUVECs, thus be physiologically significant in endothelial dysfunction. Taken together, our data suggest that Hb-AGEs may play a critical role in inducing vascular endothelial cell damage. Therefore, this study may provide a plausible explanation for the potential Hb-AGEs in human endothelial cell dysfunction of diabetic patients. © 2021 | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.relation.isPartOf | International Journal of Biological Macromolecules | - |
dc.title | Methylglyoxal-derived hemoglobin advanced glycation end products induce apoptosis and oxidative stress in human umbilical vein endothelial cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000691602200003 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2021.07.058 | - |
dc.identifier.bibliographicCitation | International Journal of Biological Macromolecules, v.187, pp.409 - 421 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85111286598 | - |
dc.citation.endPage | 421 | - |
dc.citation.startPage | 409 | - |
dc.citation.title | International Journal of Biological Macromolecules | - |
dc.citation.volume | 187 | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hyuk | - |
dc.contributor.affiliatedAuthor | Samsuzzaman, M. | - |
dc.contributor.affiliatedAuthor | Park, Sung Jean | - |
dc.contributor.affiliatedAuthor | Kim, Sun Yeou | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Endothelial dysfunction | - |
dc.subject.keywordAuthor | Hb-AGEs | - |
dc.subject.keywordAuthor | Hemoglobin | - |
dc.subject.keywordAuthor | Methylglyoxal | - |
dc.subject.keywordAuthor | ROS | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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