Brain-Derived Neurotrophic Factor Secreting Human Mesenchymal Stem Cells Improve Outcomes in Rett Syndrome Mouse Models
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyo Jeong | - |
dc.contributor.author | Bayarsaikhan, D. | - |
dc.contributor.author | Lee, Jaesuk | - |
dc.contributor.author | Bayarsaikhan, G. | - |
dc.contributor.author | Lee, Bonghee | - |
dc.date.accessioned | 2021-10-31T03:41:02Z | - |
dc.date.available | 2021-10-31T03:41:02Z | - |
dc.date.created | 2021-10-28 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 1662-4548 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82556 | - |
dc.description.abstract | Rett syndrome (RTT) is a severe X-linked dominant neurodevelopmental disorder caused by mutations in the methyl-CpG-binding protein 2 (MECP2) gene; MeCP2 regulates the expression of brain-derived neurotrophic factor (BDNF) and increasing BDNF levels ameliorates RTT symptoms. However, the clinical application of BDNF is limited, because of its short half-life and low penetrance across the blood-brain barrier. In this study, we generated BDNF-secreting mesenchymal stem cells (MSCs) from the human umbilical cord cells, using CRISPR-Cas9. We studied the effects of BDNF-MSCs in MECP2 knockout and MECP2-deficient mice. BDNF-MSCs upregulated the expression of BDNF, pAKT, and pERK1/2 and downregulated that of pp38, both in vitro and in vivo. In our in vivo experiments, BDNF-MSCs increased the body and brain weights in mice. BDNF-MSCs increased the neuronal cell numbers in the hippocampus, cortex, and striatum; in addition, they increased the number of synapses. BDNF-MSCs upregulated BDNF and the activity of BDNF downstream effectors, such as pAKT and pERK 1/2; this upregulation was persistent. In conclusion, BDNF-MSCs generated using CRISPR-Cas9 could be a therapeutic strategy for treating RTT. © Copyright © 2021 Kim, Bayarsaikhan, Lee, Bayarsaikhan and Lee. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Frontiers Media S.A. | - |
dc.relation.isPartOf | Frontiers in Neuroscience | - |
dc.title | Brain-Derived Neurotrophic Factor Secreting Human Mesenchymal Stem Cells Improve Outcomes in Rett Syndrome Mouse Models | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000708911800001 | - |
dc.identifier.doi | 10.3389/fnins.2021.725398 | - |
dc.identifier.bibliographicCitation | Frontiers in Neuroscience, v.15 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85117462289 | - |
dc.citation.title | Frontiers in Neuroscience | - |
dc.citation.volume | 15 | - |
dc.contributor.affiliatedAuthor | Kim, Hyo Jeong | - |
dc.contributor.affiliatedAuthor | Bayarsaikhan, D. | - |
dc.contributor.affiliatedAuthor | Lee, Jaesuk | - |
dc.contributor.affiliatedAuthor | Bayarsaikhan, G. | - |
dc.contributor.affiliatedAuthor | Lee, Bonghee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | brain-derived neurotrophic factor | - |
dc.subject.keywordAuthor | CRISPR-Cas system | - |
dc.subject.keywordAuthor | MECP2 | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
dc.subject.keywordAuthor | Rett syndrome | - |
dc.subject.keywordAuthor | transplantation | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | PTP1B | - |
dc.subject.keywordPlus | MICE | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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