A simple and innovative sample preparation method for on-site SARS-CoV-2 molecular diagnostics
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Songhyun | - |
dc.contributor.author | Song, Junkyu | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.accessioned | 2021-11-11T01:40:24Z | - |
dc.date.available | 2021-11-11T01:40:24Z | - |
dc.date.created | 2021-10-24 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82650 | - |
dc.description.abstract | Nucleic acid amplification is a widely used diagnostic tool, although it requires a relatively time-consuming and complicated extraction step. To address this issue outside the laboratory, we investigated a sample preparation system and determined that a silica membrane and silica-coated beads are powerful tools for the extraction from raw samples: nucleic acids are kept in the silica membrane, retained during a single wash step, and released at the elution step. The eluent is appropriate for the quantitative real-time polymerase chain reaction (qPCR) and loop-mediated amplification (LAMP) assay in terms of purity and quantity. We also built an innovative equipment-free nucleic acid extraction squeeze system which requires less than 20 min. The sample with improved purity augments the specificity and sensitivity. This system is simple, user-friendly, low-cost, and equipment-free, thus making nucleic acid extraction more accessible and affordable for researchers and untrained users. Furthermore, when combined with the reverse-transcription quantitative real-time polymerase chain reaction method, the method will accelerate the detection of diseases. The same goes when combined with the LAMP assay, especially in developing countries. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | ANALYST | - |
dc.title | A simple and innovative sample preparation method for on-site SARS-CoV-2 molecular diagnostics | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000706768300001 | - |
dc.identifier.doi | 10.1039/d1an01401c | - |
dc.identifier.bibliographicCitation | ANALYST, v.146, no.22, pp.6917 - 6923 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85118951013 | - |
dc.citation.endPage | 6923 | - |
dc.citation.startPage | 6917 | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 146 | - |
dc.citation.number | 22 | - |
dc.contributor.affiliatedAuthor | Lee, Songhyun | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article; Early Access | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | POINT | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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