Goosecoid Controls Neuroectoderm Specification via Dual Circuits of Direct Repression and Indirect Stimulation in Xenopus Embryos
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Umair, Zobia | - |
dc.contributor.author | Kumar, Vijay | - |
dc.contributor.author | Goutam, Ravi Shankar | - |
dc.contributor.author | Kumar, Shiv | - |
dc.contributor.author | Lee, Unjoo | - |
dc.contributor.author | Kim, Jaebong | - |
dc.date.accessioned | 2021-11-15T00:40:21Z | - |
dc.date.available | 2021-11-15T00:40:21Z | - |
dc.date.created | 2021-11-15 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82679 | - |
dc.description.abstract | Spemann organizer is a center of dorsal mesoderm and itself retains the mesoderm character, but it has a stimulatory role for neighboring ectoderm cells in becoming neuroectoderm in gastrula embryos. Goosecoid (Gsc) overexpression in ventral region promotes secondary axis formation including neural tissues, but the role of gsc in neural specification could be indirect. We examined the neural inhibitory and stimulatory roles of gsc in the same cell and neighboring cells contexts. In the animal cap explant system, Gsc overexpression inhibited expression of neural specific genes including foxd4l1.1, zic3, ncam, and neurod. Genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) and promoter analysis of early neural genes of foxd4l1.1 and zic3 were performed to show that the neural inhibitory mode of gsc was direct. Site-directed mutagenesis and serially deleted construct studies of foxd4l1.1 promoter revealed that Gsc directly binds within the foxd4l1.1 promoter to repress its expression. Conjugation assay of animal cap explants was also performed to demonstrate an indirect neural stimulatory role for gsc. The genes for secretory molecules, Chordin and Noggin, were up-regulated in gsc injected cells with the neural fate only achieved in gsc uninjected neighboring cells. These experiments suggested that gsc regulates neuroectoderm formation negatively when expressed in the same cell and positively in neighboring cells via soluble factors. One is a direct suppressive circuit of neural genes in gsc expressing mesoderm cells and the other is an indirect stimulatory circuit for neurogenesis in neighboring ectoderm cells via secreted BMP antagonizers. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.title | Goosecoid Controls Neuroectoderm Specification via Dual Circuits of Direct Repression and Indirect Stimulation in Xenopus Embryos | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000714815000004 | - |
dc.identifier.doi | 10.14348/molcells.2021.0055 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.44, no.10, pp.723 - 735 | - |
dc.identifier.kciid | ART002769101 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85120780874 | - |
dc.citation.endPage | 735 | - |
dc.citation.startPage | 723 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 44 | - |
dc.citation.number | 10 | - |
dc.contributor.affiliatedAuthor | Umair, Zobia | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | element | - |
dc.subject.keywordAuthor | neuroectoderm | - |
dc.subject.keywordAuthor | Noggin | - |
dc.subject.keywordAuthor | transcriptional regulation | - |
dc.subject.keywordAuthor | Xenopus | - |
dc.subject.keywordPlus | IMMATURE NEURAL ECTODERM | - |
dc.subject.keywordPlus | SPEMANN ORGANIZER | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL REPRESSION | - |
dc.subject.keywordPlus | MARGINAL ZONE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | CHORDIN | - |
dc.subject.keywordPlus | FOX | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BRACHYURY | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.