Identification of Renoprotective Phytosterols from Mulberry (Morus alba) Fruit against Cisplatin-Induced Cytotoxicity in LLC-PK1 Kidney Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Dahae | - |
dc.contributor.author | Lee, Seoung Rak | - |
dc.contributor.author | Park, Bang Ju | - |
dc.contributor.author | Song, Ji Hoon | - |
dc.contributor.author | Kim, Jung Kyu | - |
dc.contributor.author | Ko, Yuri | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.contributor.author | Kim, Ki Hyun | - |
dc.date.accessioned | 2021-12-16T01:40:32Z | - |
dc.date.available | 2021-12-16T01:40:32Z | - |
dc.date.created | 2021-11-21 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 2223-7747 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82945 | - |
dc.description.abstract | The aim of this study was to explore the protective effects of bioactive compounds from the fruit of the mulberry tree (Morus alba L.) against cisplatin-induced apoptosis in LLC-PK1 pig kidney epithelial cells. Morus alba fruit is a well-known edible fruit commonly used in traditional folk medicine. Chemical investigation of M. alba fruit resulted in the isolation and identification of six phytosterols (1–6). Their structures were determined as 7-ketositosterol (1), stigmast-4-en-3β-ol-6-one (2), (3β,6α)-stigmast-4-ene-3,6-diol (3), stigmast-4-ene-3β,6β-diol (4), 7β-hydroxysitosterol 3-O-β-D-glucoside (5), and 7α-hydroxysitosterol 3-O-β-D-glucoside (6) by analyzing their physical and spectroscopic data as well as liquid chromatography/mass spectrometry data. All compounds displayed protective effects against cisplatin-induced LLC-PK1 cell damage, improving cisplatin-induced cytotoxicity to more than 80% of the control value. Compound 1 displayed the best effect at a relatively low concentration by inhibiting the percentage of apoptotic cells following cisplatin treatment. Its molecular mechanisms were identified using Western blot assays. Treatment of LLC-PK1 cells with compound 1 decreased the upregulated phosphorylation of p38 and c-Jun N-terminal kinase (JNK) following cisplatin treatment. In addition, compound 1 significantly suppressed cleaved caspase-3 in cisplatin-induced LLC-PK1 cells. Taken together, these findings indicated that cisplatin-induced apoptosis was significantly inhibited by compound 1 in LLC-PK1 cells, thereby supporting the potential of 7-ketositosterol (1) as an adjuvant candidate for treating cisplatin-induced nephrotoxicity. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Plants | - |
dc.title | Identification of Renoprotective Phytosterols from Mulberry (Morus alba) Fruit against Cisplatin-Induced Cytotoxicity in LLC-PK1 Kidney Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000727888700001 | - |
dc.identifier.doi | 10.3390/plants10112481 | - |
dc.identifier.bibliographicCitation | Plants, v.10, no.11 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85119076222 | - |
dc.citation.title | Plants | - |
dc.citation.volume | 10 | - |
dc.citation.number | 11 | - |
dc.contributor.affiliatedAuthor | Lee, Dahae | - |
dc.contributor.affiliatedAuthor | Park, Bang Ju | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | LLC-PK1 | - |
dc.subject.keywordAuthor | MAPKs | - |
dc.subject.keywordAuthor | Morus alba | - |
dc.subject.keywordAuthor | Mulberry | - |
dc.subject.keywordAuthor | Nephrotoxicity | - |
dc.subject.keywordAuthor | Phytosterols | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | NEPHROTOXICITY | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | MUSHROOM | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | STEROLS | - |
dc.subject.keywordPlus | SEEDS | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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