Salvia miltiorrhiza Prevents Methylglyoxal-Induced Glucotoxicity via the Regulation of Apoptosis-Related Pathways and the Glyoxalase System in Human Umbilical Vein Endothelial Cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Jae Sung | - |
dc.contributor.author | Lee, Jae Hyuk | - |
dc.contributor.author | Hong, Seong Min | - |
dc.contributor.author | Cho, Kyo Hee | - |
dc.contributor.author | Kim, Sun Yeou | - |
dc.date.accessioned | 2022-01-14T01:40:18Z | - |
dc.date.available | 2022-01-14T01:40:18Z | - |
dc.date.created | 2022-01-14 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83261 | - |
dc.description.abstract | Methylglyoxal (MGO), which is produced as a byproduct of glucose metabolism, is the leading to diabetic cardiovascular complications. Salvia miltiorrhiza Bunge (Lamiaceae) has been reported as a potential plant to control diabetes and cardiovascular disease. However, no report exists on the effect of Salvia miltiorrhiza Bunge extract (SME) on MGO-induced glucotoxicity in human umbilical vein endothelial cells (HUVECs). We demonstrated the protective effects of SME (1, 5, and 10 mu g/mL) and its components against MGO-induced endothelial dysfunction in HUVECs. Cytotoxicity was evaluated using the several in vitro experiments. Additionally, the protein expression of receptor of advanced glycation end-products (RAGE), mitogen-activated protein kinase (MAPK) pathway and glyoxalase system were measured. Then, the inhibitory effects of SME and its main components on MGO-induced oxidative stress, radical scavenging, formation of MGO-derived advanced glycation end products (AGEs), and MGO-AGEs crosslinking were evaluated. SME (10 mu g/mL) strongly prevented expressed levels of RAGE, MGO-induced apoptosis and reduced reactive oxygen species (ROS) generation in HUVECs, comparing with 1 mM aminoguanidine. Additionally, SME (5 and 10 mu g/mL) reduced the expression of proteins (e.g., p-extracellular signal-regulated kinase (ERK) and p-p38) in the MAPKs pathway and upregulated the glyoxalase system in HUVECs. SME (0.5-10 mg/mL), dihydrotanshinone (0.4 mM), and rosmarinic acid (0.4 mM) prevented MGO-AGEs formation and broke the MGO-AGE crosslinking. These results show that S. miltiorrhiza has protective effects against MGO-induced glucotoxicity by regulating the proteins involved in apoptosis, glyoxalase system and antioxidant activity. We expect that S. miltiorrhiza is a potential natural resource for the treatment of MGO-induced vascular endothelial dysfunction. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.relation.isPartOf | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.title | Salvia miltiorrhiza Prevents Methylglyoxal-Induced Glucotoxicity via the Regulation of Apoptosis-Related Pathways and the Glyoxalase System in Human Umbilical Vein Endothelial Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000740637200006 | - |
dc.identifier.doi | 10.1248/bpb.b21-00507 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.45, no.1, pp.51 - 62 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85123169088 | - |
dc.citation.endPage | 62 | - |
dc.citation.startPage | 51 | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 45 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Jae Sung | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hyuk | - |
dc.contributor.affiliatedAuthor | Hong, Seong Min | - |
dc.contributor.affiliatedAuthor | Cho, Kyo Hee | - |
dc.contributor.affiliatedAuthor | Kim, Sun Yeou | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Salvia miltiorrhiza extract | - |
dc.subject.keywordAuthor | methylglyoxal | - |
dc.subject.keywordAuthor | advanced glycation end-product | - |
dc.subject.keywordAuthor | endothelial dysfunction | - |
dc.subject.keywordAuthor | human umbilical vein endothelial cell | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordPlus | GLYCATION END-PRODUCTS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | TANSHINONE IIA | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.subject.keywordPlus | DEFENSE | - |
dc.subject.keywordPlus | ACID | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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