Estrogenic Activity of Mycoestrogen (3β,5α,22E)-Ergost-22-en-3-ol via Estrogen Receptor α-Dependent Signaling Pathways in MCF-7 Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Dahae | - |
dc.contributor.author | Ko, Yuri | - |
dc.contributor.author | Pang, Changhyun | - |
dc.contributor.author | Ko, Yoon-Joo | - |
dc.contributor.author | Choi, You-Kyoung | - |
dc.contributor.author | Kim, Ki Hyun | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.date.accessioned | 2022-02-03T01:40:44Z | - |
dc.date.available | 2022-02-03T01:40:44Z | - |
dc.date.created | 2022-01-19 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83405 | - |
dc.description.abstract | Armillariella tabescens (Scop.) Sing., a mushroom of the family Tricholomataceae, has been used in traditional oriental medicine to treat cholecystitis, improve bile secretion, and regulate bile-duct pressure. The present study evaluated the estrogen-like effects of A. tabescens using a cell-proliferation assay in an estrogen-receptor-positive breast cancer cell line (MCF-7). We found that the methanol extract of A. tabescens fruiting bodies promoted cell proliferation in MCF-7 cells. Using bioassay-guided fractionation of the methanol extract and chemical investigation, we isolated and identified four steroids and four fatty acids from the active fraction. All eight compounds were evaluated by E-screen assay for their estrogen-like effects in MCF-7 cells. Among the tested isolates, only (3β,5α,22E)-ergost-22-en-3-ol promoted cell proliferation in MCF-7 cells; this effect was mitigated by the ER antagonist, ICI 182,780. The mechanism underlying the estrogen-like effect of (3β,5α,22E)ergost-22-en-3-ol was evaluated using Western blot analysis to detect the expression of extracellular signal-regulated kinase (ERK), phosphatidylinositol 3-kinase (PI3K), Akt, and estrogen receptor α (ERα). We found that (3β,5α,22E)-ergost-22-en-3-ol induced an increase in phosphorylation of ERK, PI3K, Akt, and ERα. Together, these experimental results suggest that (3β,5α,22E)-ergost-22-en-3-ol is responsible for the estrogen-like effects of A. tabescens and may potentially aid control of estrogenic activity in menopause. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Molecules | - |
dc.title | Estrogenic Activity of Mycoestrogen (3β,5α,22E)-Ergost-22-en-3-ol via Estrogen Receptor α-Dependent Signaling Pathways in MCF-7 Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000743717700001 | - |
dc.identifier.doi | 10.3390/molecules27010036 | - |
dc.identifier.bibliographicCitation | Molecules, v.27, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85121660180 | - |
dc.citation.title | Molecules | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Lee, Dahae | - |
dc.contributor.affiliatedAuthor | Choi, You-Kyoung | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | (3β,5α,22E)-ergost-22-en-3-ol | - |
dc.subject.keywordAuthor | Armillariella tabescens | - |
dc.subject.keywordAuthor | Estrogen receptor | - |
dc.subject.keywordAuthor | Phytoestrogens | - |
dc.subject.keywordPlus | FRUITING BODIES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PHYTOESTROGENS | - |
dc.subject.keywordPlus | ZEARALENONE | - |
dc.subject.keywordPlus | ANTITUMOR | - |
dc.subject.keywordPlus | MUSHROOM | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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