Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode
DC Field | Value | Language |
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dc.contributor.author | Le, Hien T. Ngoc | - |
dc.contributor.author | Cho, Sungbo | - |
dc.date.accessioned | 2022-02-04T00:40:41Z | - |
dc.date.available | 2022-02-04T00:40:41Z | - |
dc.date.created | 2022-01-19 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 2227-9059 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83422 | - |
dc.description.abstract | The development of an electrochemical biosensor for the detection of phosphorylated-tau threonine 231 (p-tau231), a biomarker of Alzheimer’s disease (AD), has yet to be achieved. Therefore, in this study, we developed a simple, small size, cheap, and sensitive electrochemical biosensor based on an interdigitated wave-shaped electrode via an activated self-assembled monolayer to preserve a specific anti–p-tau231 antibody (IWE/SAM/EDC-NHS/anti–p-tau231). Detection of p-tau231 in human serum (HS) using the biosensor was undertaken using electrochemical impedance spectroscopy (EIS). The change in charge-transfer resistance (Rct) in the EIS analysis of the biosensor indicated the detection of p-tau231 in HS within a wide linear range of detection (10−4 –101 ng mL−1), and a low limit of detection (140 pg mL−1). This lower limit is less than the detection level of p-tau231 in cerebrospinal fluid (CSF) (700 pg mL−1) of AD patients and the level of CSF p-tau231 of patients with mild cognitive impairment (501 pg mL−1), demonstrating the possibility of using the biosensor in detection of p-tau231 at early stage AD. A high binding affinity and low dissociation constant (Kd) between anti–p-tau231 and p-tau231 in HS was demonstrated by using a biosensor and Kd was 7.6 pM, demonstrating the high specific detection of p-tau231 by the biosensor. The good selectivity of the biosensor for the detection of p-tau231 with differential analytes was also examined in this study. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Biomedicines | - |
dc.title | Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000747618500001 | - |
dc.identifier.doi | 10.3390/biomedicines10010010 | - |
dc.identifier.bibliographicCitation | Biomedicines, v.10, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85122230206 | - |
dc.citation.title | Biomedicines | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Le, Hien T. Ngoc | - |
dc.contributor.affiliatedAuthor | Cho, Sungbo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Alzheimer’s disease | - |
dc.subject.keywordAuthor | Binding affinity | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Electrochemical impedance spectroscopy | - |
dc.subject.keywordAuthor | Micro-interdigitated electrode | - |
dc.subject.keywordAuthor | Phosphorylated-tau threonine 231 | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | SUPRAMOLECULAR RECOGNITION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | GOLD | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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