Ethanol Extract of Elaeagnus glabra f. oxyphylla Branches Alleviates the Inflammatory Response Through Suppression of Cyclin D3/Cyclin-Dependent Kinase 11(p58) Coupled to Lipopolysaccharide-Activated BV-2 Microglia
DC Field | Value | Language |
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dc.contributor.author | Lim, Hye-Sun | - |
dc.contributor.author | Sohn, Eunjin | - |
dc.contributor.author | Kim, Yu Jin | - |
dc.contributor.author | Kim, Bu-Yeo | - |
dc.contributor.author | Kim, Joo-Hwan | - |
dc.contributor.author | Jeong, Soo-Jin | - |
dc.date.accessioned | 2022-02-12T01:40:12Z | - |
dc.date.available | 2022-02-12T01:40:12Z | - |
dc.date.created | 2022-02-12 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 1934-578X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83467 | - |
dc.description.abstract | Neuroinflammation plays a pivotal role in the pathogenesis of neurodegenerative diseases and is characterized by microglial dysregulation. Here, we explored the beneficial effects of a leaf extract of Elaeagnus glabra f. oxyphylla (EGFO), a native medicinal plant to Korea, South China, Japan, and Taiwan, on neuroinflammation using lipopolysaccharide (LPS)-stimulated BV-2 microglia. Levels of the inflammatory mediators were determined by enzyme-linked immunosorbent assays and reverse transcription-polymerase chain reaction. The phospho levels of mitogen-activated protein kinases, which are key kinase molecules in the inflammatory signaling pathway in microglia, were analyzed by Western blotting. Treatment with EGFO significantly suppressed the LPS-mediated induction of nitric oxide and prostaglandin E-2. Consistently, EGFO treatment in LPS-stimulated BV-2 cells markedly reduced the inflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels. The best concentration of EGFO that could reduce TNF-alpha and IL-6 was 100 mu g/mL. EGFO relatively reduced the messenger RNA expression of TNF-alpha and IL-6 by 0.36 and 0.32-fold ratio, respectively, compared to LPS treatment. Moreover, EGFO markedly reduced the phospho levels of p38 and the c-jun N-terminal kinase. Furthermore, antibody microarray and immunoblotting data revealed that the pharmacological mechanisms driving the antineuroinflammatory action of EGFO involve prevention of the cyclin D3/cyclin-dependent kinase 11(p58) (CDK11(p58)) interaction. In conclusion, our results demonstrate that EGFO alleviates the inflammatory response through the suppression of cyclin D3/CDK11(p58) coupling in LPS-activated BV-2 microglia. We propose the potential of EGFO as a novel drug candidate for neurodegenerative diseases by targeting neuroinflammation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.relation.isPartOf | NATURAL PRODUCT COMMUNICATIONS | - |
dc.title | Ethanol Extract of Elaeagnus glabra f. oxyphylla Branches Alleviates the Inflammatory Response Through Suppression of Cyclin D3/Cyclin-Dependent Kinase 11(p58) Coupled to Lipopolysaccharide-Activated BV-2 Microglia | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000751634900001 | - |
dc.identifier.doi | 10.1177/1934578X221075079 | - |
dc.identifier.bibliographicCitation | NATURAL PRODUCT COMMUNICATIONS, v.17, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85124421910 | - |
dc.citation.title | NATURAL PRODUCT COMMUNICATIONS | - |
dc.citation.volume | 17 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Joo-Hwan | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | antibody array | - |
dc.subject.keywordAuthor | cyclin D3 | - |
dc.subject.keywordAuthor | cyclin-dependent kinase 11(p58) | - |
dc.subject.keywordAuthor | Elaeagnus glabra f | - |
dc.subject.keywordAuthor | oxyphylla | - |
dc.subject.keywordAuthor | microglia | - |
dc.subject.keywordAuthor | neuroinflammation | - |
dc.subject.keywordAuthor | neurodegenerative diseases | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | INDUCED NEUROINFLAMMATION | - |
dc.subject.keywordPlus | BV2 MICROGLIA | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | P38 | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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