Wogonin inhibits tight junction disruption via suppression of inflammatory response and phosphorylation of AKT/NF-kappa B and ERK1/2 in rhinovirus-infected human nasal epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyeong Ah | - |
dc.contributor.author | Jung, Joo Hyun | - |
dc.contributor.author | Choi, Yun Sook | - |
dc.contributor.author | Kim, Seon Tae | - |
dc.date.accessioned | 2022-03-22T04:40:17Z | - |
dc.date.available | 2022-03-22T04:40:17Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83784 | - |
dc.description.abstract | Objective The maintenance of tight junction integrity contributes significantly to epithelial barrier function. If barrier function is destroyed, cell permeability increases and the movement of pathogens is promoted, further increasing the susceptibility to secondary infection. Here, we examined the protective effects of wogonin on rhinovirus (RV)-induced tight junction disruption. Additionally, we examined the signaling molecules responsible for anti-inflammatory activities in human nasal epithelial (HNE) cells. Methods and results Primary HNE cells grown at an air-liquid interface and RPMI 2650 cells were infected apically with RV. Incubation with RV resulted in disruption of tight junction proteins (ZO-1, E-cadherin, claudin-1, and occludin) in the HNE cells. Cell viability of wogonin-treated HNE cells was measured using the MTT assay. Pretreatment with wogonin decreased RV-induced disruption of tight junctions in HNE cells. Furthermore, wogonin significantly decreased RV-induced phosphorylation of Akt/NF-kappa B and ERK1/2. Additionally, RV-induced generation of reactive oxygen species and RV-induced up-regulation of the production of inflammatory cytokines IL-8 and IL-6 were diminished by wogonin in HNE cells. Conclusion Wogonin inhibits HRV-induced tight junction disruption via the suppression of inflammatory responses and phosphorylation of Akt/NF-kappa B and ERK1/2 in HNE cells. These finds will facilitate the development of novel therapeutic strategies. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER BASEL AG | - |
dc.relation.isPartOf | INFLAMMATION RESEARCH | - |
dc.title | Wogonin inhibits tight junction disruption via suppression of inflammatory response and phosphorylation of AKT/NF-kappa B and ERK1/2 in rhinovirus-infected human nasal epithelial cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000749947800001 | - |
dc.identifier.doi | 10.1007/s00011-022-01542-w | - |
dc.identifier.bibliographicCitation | INFLAMMATION RESEARCH, v.71, no.3, pp.357 - 368 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85124125214 | - |
dc.citation.endPage | 368 | - |
dc.citation.startPage | 357 | - |
dc.citation.title | INFLAMMATION RESEARCH | - |
dc.citation.volume | 71 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Kim, Kyeong Ah | - |
dc.contributor.affiliatedAuthor | Jung, Joo Hyun | - |
dc.contributor.affiliatedAuthor | Choi, Yun Sook | - |
dc.contributor.affiliatedAuthor | Kim, Seon Tae | - |
dc.type.docType | Article; Early Access | - |
dc.subject.keywordAuthor | Wogonin | - |
dc.subject.keywordAuthor | Rhinovirus | - |
dc.subject.keywordAuthor | HNE cells | - |
dc.subject.keywordAuthor | Tight junction proteins | - |
dc.subject.keywordPlus | SCUTELLARIA | - |
dc.subject.keywordPlus | P38 | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | MAPK | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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