TGF-β activates NLRP3 inflammasome by an autocrine production of TGF-β in LX-2 human hepatic stellate cells
DC Field | Value | Language |
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dc.contributor.author | Kang, Hwansu | - |
dc.contributor.author | Seo, Eunhui | - |
dc.contributor.author | Oh, Yoon Sin | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.date.accessioned | 2022-04-12T08:40:04Z | - |
dc.date.available | 2022-04-12T08:40:04Z | - |
dc.date.created | 2022-02-20 | - |
dc.date.issued | 2022-05 | - |
dc.identifier.issn | 0300-8177 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83939 | - |
dc.description.abstract | Inflammation contributes to the pathogenesis of liver disease, and inflammasome activation has been identified as a major contributor to the amplification of liver inflammation. Transforming growth factor-beta (TGF-β) is a key regulator of liver physiology, contributing to all stages of liver disease. We investigated whether TGF-β is involved in inflammasome-mediated fibrosis in hepatic stellate cells. Treatment with TGF-β increased priming of NLRP3 inflammasome signaling by increasing NLRP3 levels and activating TAK1-NF-kB signaling. Moreover, TGF-β increased the expression of p-Smad2/3-NOX4 in LX-2 cells and consequently increased ROS content, which is a trigger for NLRP3 inflammasome activation. Elevated expression of NEK7 and active caspase-1 was also shown in TGF-β-induced LX-2 cells, and this level was reduced by (5Z)-oxozeaenol, a TAK inhibitor. Finally, TGF-β-treated cells significantly increased TGF-β secretion levels, and their production was inhibited by IL-1β receptor antagonist treatment. In conclusion, TGF-β may represent an endogenous danger signal to the active NLRP3 inflammasome, by which IL-1β mediates TGF-β expression in an autocrine manner. Therefore, targeting the NLRP3 inflammasome may be a promising approach for the development of therapies for TGF-β-induced liver fibrosis. © 2022, The Author(s). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Springer | - |
dc.relation.isPartOf | Molecular and Cellular Biochemistry | - |
dc.title | TGF-β activates NLRP3 inflammasome by an autocrine production of TGF-β in LX-2 human hepatic stellate cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000753223800002 | - |
dc.identifier.doi | 10.1007/s11010-022-04369-5 | - |
dc.identifier.bibliographicCitation | Molecular and Cellular Biochemistry, v.477, no.5, pp.1329 - 1338 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85124551908 | - |
dc.citation.endPage | 1338 | - |
dc.citation.startPage | 1329 | - |
dc.citation.title | Molecular and Cellular Biochemistry | - |
dc.citation.volume | 477 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Kang, Hwansu | - |
dc.contributor.affiliatedAuthor | Seo, Eunhui | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article; Early Access | - |
dc.subject.keywordAuthor | Hepatic stellate cells | - |
dc.subject.keywordAuthor | Liver fibrosis | - |
dc.subject.keywordAuthor | NLRP3 inflammasome | - |
dc.subject.keywordAuthor | TGF-β | - |
dc.subject.keywordPlus | TAK1 | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | IL-1-BETA | - |
dc.subject.keywordPlus | MEDIATOR | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordPlus | NEK7 | - |
dc.subject.keywordPlus | ATP | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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