Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yousuf, Mohd | - |
dc.contributor.author | Ali, Asghar | - |
dc.contributor.author | Khan, Parvez | - |
dc.contributor.author | Anjum, Farah | - |
dc.contributor.author | Elasbali, Abdelbaset Mohamed | - |
dc.contributor.author | Islam, Asimul | - |
dc.contributor.author | Yadav, Dharmendra Kumar | - |
dc.contributor.author | Shafie, Alaa | - |
dc.contributor.author | Haque, Qazi Mohd Rizwanul | - |
dc.contributor.author | Hassan, Md Imtaiyaz | - |
dc.date.accessioned | 2022-04-13T03:40:22Z | - |
dc.date.available | 2022-04-13T03:40:22Z | - |
dc.date.created | 2022-04-13 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 2076-2607 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83966 | - |
dc.description.abstract | Background: Prolactin inducible protein (PIP) is a small secretary glycoprotein present in most biological fluids and contributes to various cellular functions, including cell growth, fertility, antitumor, and antifungal activities. Objectives: The present study evaluated the antibacterial activities of recombinant PIP against multiple broad-spectrum MDR bacterial strains. Methods: The PIP gene was cloned, expressed and purified using affinity chromatography. Disk diffusion, broth microdilution, and growth kinetic assays were used to determine the antibacterial activities of PIP. Results: Disk diffusion assay showed that PIP has a minimum and maximum zone of inhibition against E. coli and P. aeruginosa, respectively, compared to the reference drug ampicillin. Furthermore, growth kinetics studies also suggested that PIP significantly inhibited the growth of E. coli and P. aeruginosa. The minimum inhibitory concentration of PIP was 32 mu g/mL for E. coli (443), a standard bacterial strain, and 64 mu g/mL for Bacillus sp. (LG1), an environmental multidrug-resistant (MDR) strain. The synergistic studies of PIP with ampicillin showed better efficacies towards selected bacterial strains having MDR properties. Conclusion: Our findings suggest that PIP has a broad range of antibacterial activities with important implications in alleviating MDR problems. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MICROORGANISMS | - |
dc.title | Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000774068200001 | - |
dc.identifier.doi | 10.3390/microorganisms10030597 | - |
dc.identifier.bibliographicCitation | MICROORGANISMS, v.10, no.3 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85126069003 | - |
dc.citation.title | MICROORGANISMS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Yadav, Dharmendra Kumar | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | prolactin inducible protein | - |
dc.subject.keywordAuthor | antibacterial activity | - |
dc.subject.keywordAuthor | multidrug-resistant bacteria | - |
dc.subject.keywordAuthor | small proteins | - |
dc.subject.keywordPlus | HUMAN SEMINAL PLASMA | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | EP-GP | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | GLYCOPROTEIN | - |
dc.subject.keywordPlus | PIP | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | GCDFP-15 | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.