Dual Beneficial Effects of alpha-Spinasterol Isolated from Aster pseudoglehnii on Glucose Uptake in Skeletal Muscle Cells and Glucose-Stimulated Insulin Secretion in Pancreatic beta-Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Dahae | - |
dc.contributor.author | Kim, Ji-Young | - |
dc.contributor.author | Kwon, Hak Cheol | - |
dc.contributor.author | Kwon, Jaeyoung | - |
dc.contributor.author | Jang, Dae Sik | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.date.accessioned | 2022-04-19T08:40:30Z | - |
dc.date.available | 2022-04-19T08:40:30Z | - |
dc.date.created | 2022-04-19 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 2223-7747 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84062 | - |
dc.description.abstract | Herein, we determined whether alpha-Spinasterol, a stigmastane-type phytosterol isolated from Aster pseudoglehnii, potentially impacts glucose uptake and glucose-stimulated insulin secretion in skeletal muscle cells and pancreatic beta-cells, respectively. We observed that A. pseudoglehnii and its fractions enhanced glucose uptake, with no toxic effects on C2C12 cells, with the n-hexane fraction exhibiting the most potent effect. alpha-Spinasterol, isolated from the n-hexane fraction, enhanced glucose uptake with no toxic effects on C2C12 cells. Additionally, alpha-Spinasterol increased the expression of associated proteins, including insulin receptor substrate-1, AMP-activated protein kinase, and glucose transporter type 4, as determined by Western blotting. Furthermore, alpha-Spinasterol enhanced insulin secretion in response to high glucose concentrations, with no toxic effects on INS-1 cells; this effect was superior to that demonstrated by gliclazide (positive control), commonly prescribed to treat type 2 diabetes (T2D). alpha-Spinasterol enhanced the expression of associated proteins, including insulin receptor substrate-2, peroxisome proliferator-activated receptor gamma, and pancreatic and duodenal homeobox 1, as determined using Western blotting. The insulin secretory effect of alpha-Spinasterol was enhanced by a K+ channel blocker and L-type Ca2+ channel agonist and was suppressed by a K+ channel activator and L-type Ca2+ channel blocker. alpha-Spinasterol isolated from A. pseudoglehnii may improve hyperglycemia by improving glucose uptake into skeletal muscle cells and enhancing insulin secretion in pancreatic beta-cells. Accordingly, alpha-Spinasterol could be a potential candidate for anti-T2D therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | PLANTS-BASEL | - |
dc.title | Dual Beneficial Effects of alpha-Spinasterol Isolated from Aster pseudoglehnii on Glucose Uptake in Skeletal Muscle Cells and Glucose-Stimulated Insulin Secretion in Pancreatic beta-Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000772925000001 | - |
dc.identifier.doi | 10.3390/plants11050658 | - |
dc.identifier.bibliographicCitation | PLANTS-BASEL, v.11, no.5 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85125286222 | - |
dc.citation.title | PLANTS-BASEL | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Lee, Dahae | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Aster pseudoglehnii | - |
dc.subject.keywordAuthor | alpha-Spinasterol | - |
dc.subject.keywordAuthor | glucose-stimulated insulin secretion | - |
dc.subject.keywordAuthor | glucose uptake | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | HYPERGLYCEMIA | - |
dc.subject.keywordPlus | COMBINATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GINSENG | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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