Fabrication of a fully integrated paper microdevice for point-of-care testing of infectious disease using Safranin O dye coupled with loop-mediated isothermal amplification
DC Field | Value | Language |
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dc.contributor.author | Dinh, Vu Phong | - |
dc.contributor.author | Lee, Nae Yoon | - |
dc.date.accessioned | 2022-04-28T02:40:16Z | - |
dc.date.available | 2022-04-28T02:40:16Z | - |
dc.date.created | 2022-02-19 | - |
dc.date.issued | 2022-05-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84118 | - |
dc.description.abstract | In this study, we introduce a paper microdevice fully integrating DNA extraction, loop-mediated isothermal amplification (LAMP), and Safranin O-based colorimetric detection of two major infectious pathogens, namely SARS-CoV-2 and Enterococcus faecium. The paper microdevice is composed of two parts: sample and reaction chambers. A sealing film acted as a bottom layer to allow foldable motion for transferring DNA from sample chamber to reaction chamber in a seamless manner. An FTA card was employed in the sample chamber for DNA extraction and purification from bacteria-spiked milk. After LAMP reaction at 65 °C for 30 min, a novel aggregation-based DNA detection was obtained by Safranin O polymerization in the reaction chamber. Specifically, Safranin O underwent polymerization by addition of oxidant to form Safranin O oligomers. The electrostatic interaction between the positively charged Safranin O oligomers and the negatively charged DNA comprising LAMP amplicons resulted in the aggregation with a dark red color. Meanwhile, in the absence of LAMP amplicons, Safranin O oligomers were well dispersed and displayed their original red color. By using Safranin O-based detection, SARS-CoV-2 and E. faecium were successfully identified by naked eye within 60 min, and the limits of detection were 10-4 ng/μL and 102 CFU/mL, respectively. These results indicate that a fully integrated paper microdevice plays an important role in sample-in-answer-out format in the genetic analyses of infectious disease and serves as a rapid tool for controlling the spread of diseases. © 2022 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.title | Fabrication of a fully integrated paper microdevice for point-of-care testing of infectious disease using Safranin O dye coupled with loop-mediated isothermal amplification | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000782659400007 | - |
dc.identifier.doi | 10.1016/j.bios.2022.114080 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.204 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85124461723 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 204 | - |
dc.contributor.affiliatedAuthor | Dinh, Vu Phong | - |
dc.contributor.affiliatedAuthor | Lee, Nae Yoon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Aggregation-based DNA detection | - |
dc.subject.keywordAuthor | Fully integrated paper microdevice | - |
dc.subject.keywordAuthor | Infectious disease | - |
dc.subject.keywordAuthor | Multiple pathogen detection | - |
dc.subject.keywordAuthor | Point-of-care testing | - |
dc.subject.keywordAuthor | Safranin O | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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