Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Choon Guen | - |
dc.contributor.author | Lee, Jisun | - |
dc.contributor.author | Lee, Da Gyung | - |
dc.contributor.author | Kim, Joo Won | - |
dc.contributor.author | Alnaeeli, Mawadda | - |
dc.contributor.author | Park, Yong Il | - |
dc.contributor.author | Park, Jae Kweon | - |
dc.date.available | 2020-02-28T02:42:54Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8414 | - |
dc.description.abstract | A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed. The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars. However, glycerol and xylitol were identified as the main sugar alcohols. Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups. MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization. Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-alpha and IL-6 in a dose-dependent manner. Furthermore, treatment of RAW 264.7 cells with PAL for 24 h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged. These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway. To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | XYLITOL PRODUCTION | - |
dc.subject | IMMUNE-RESPONSE | - |
dc.subject | MACROPHAGES | - |
dc.subject | ACTIVATION | - |
dc.subject | SWEETENERS | - |
dc.subject | SURVIVAL | - |
dc.subject | BACTERIA | - |
dc.subject | SYSTEM | - |
dc.title | Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000370886800059 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2016.01.027 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.85, pp.505 - 513 | - |
dc.identifier.scopusid | 2-s2.0-84954339096 | - |
dc.citation.endPage | 513 | - |
dc.citation.startPage | 505 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 85 | - |
dc.contributor.affiliatedAuthor | Lee, Choon Guen | - |
dc.contributor.affiliatedAuthor | Lee, Da Gyung | - |
dc.contributor.affiliatedAuthor | Kim, Joo Won | - |
dc.contributor.affiliatedAuthor | Park, Jae Kweon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Taxus cuspidata | - |
dc.subject.keywordAuthor | Polyhydric alcohol | - |
dc.subject.keywordAuthor | Immune-stimulation | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | XYLITOL PRODUCTION | - |
dc.subject.keywordPlus | IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SWEETENERS | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.