Bacteroides fragilis Toxin Induces Intestinal Epithelial Cell Secretion of Interleukin-8 by the E-Cadherin/beta-Catenin/NF-kappa B Dependent Pathway
DC Field | Value | Language |
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dc.contributor.author | Lee, Chang-Gun | - |
dc.contributor.author | Hwang, Soonjae | - |
dc.contributor.author | Gwon, Sun-Yeong | - |
dc.contributor.author | Park, Chanoh | - |
dc.contributor.author | Jo, Minjeong | - |
dc.contributor.author | Hong, Ju-Eun | - |
dc.contributor.author | Rhee, Ki-Jong | - |
dc.date.accessioned | 2022-04-30T02:40:20Z | - |
dc.date.available | 2022-04-30T02:40:20Z | - |
dc.date.created | 2022-04-30 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 2227-9059 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84167 | - |
dc.description.abstract | Enterotoxigenic Bacteroides fragilis (ETBF) has emerged as a gut microbiome pathogen that can promote colitis associated cancer in humans. ETBF secretes the metalloprotease, B. fragilis toxin (BFT), which can induce ectodomain cleavage of E-cadherin and IL-8 secretion through the beta-catenin, NF-kappa B, and MAPK pathways in intestinal epithelial cells. However, it is still unclear whether E-cadherin cleavage is required for BFT induced IL-8 secretion and the relative contribution of these signaling pathways to IL-8 secretion. Using siRNA knockdown and CRISPR knockout studies, we found that E-cadherin cleavage is required for BFT mediated IL-8 secretion. In addition, genetic ablation of 13-catenin indicates that beta-catenin is required for the BFT induced increase in transcriptional activity of NF-kappa B, p65 nuclear localization and early IL-8 secretion. These results suggest that BFT induced beta-catenin signaling is upstream of NF-kappa B activation. However, despite beta-catenin gene disruption, BFT still activated the MAPK pathway, suggesting that the BFT induced activation of the MAPK signaling pathway is independent from the E-cadherin/beta-catenin/NF-kappa B pathway. These findings show that E-cadherin and beta-catenin play a critical role in acute inflammation following ETBF infection through the inflammatory response to BFT in intestinal epithelial cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | BIOMEDICINES | - |
dc.title | Bacteroides fragilis Toxin Induces Intestinal Epithelial Cell Secretion of Interleukin-8 by the E-Cadherin/beta-Catenin/NF-kappa B Dependent Pathway | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000785326600001 | - |
dc.identifier.doi | 10.3390/biomedicines10040827 | - |
dc.identifier.bibliographicCitation | BIOMEDICINES, v.10, no.4 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85128411086 | - |
dc.citation.title | BIOMEDICINES | - |
dc.citation.volume | 10 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Hwang, Soonjae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | enterotoxigenic Bacteroides fragilis | - |
dc.subject.keywordAuthor | colonic epithelial cell | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.subject.keywordAuthor | beta-catenin | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | ENTEROTOXIN | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | CONTRIBUTES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | COLITIS | - |
dc.subject.keywordPlus | MAPK | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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