Live isolation of naïve ESCs via distinct glucose metabolism and stored glycogen
DC Field | Value | Language |
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dc.contributor.author | Kim, Keun-Tae | - |
dc.contributor.author | Oh, Ji-Young | - |
dc.contributor.author | Park, Seokwoo | - |
dc.contributor.author | Kim, Seong-Min | - |
dc.contributor.author | Benjamin, P. | - |
dc.contributor.author | Park, In-Hyun | - |
dc.contributor.author | Chun, Kwang-Hoon | - |
dc.contributor.author | Chang, Young-Tae | - |
dc.contributor.author | Cha, Hyuk-Jin | - |
dc.date.accessioned | 2022-05-22T06:40:05Z | - |
dc.date.available | 2022-05-22T06:40:05Z | - |
dc.date.created | 2022-05-04 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84386 | - |
dc.description.abstract | Naïve and primed pluripotent stem cells recapitulate the peri- and post-implantation development, respectively. Thus, investigation of distinct traits between each pluripotent stem cell type would shed light on early embryonic processes. Herein, by screening a fluorescent probe library, we found that intracellular glycogen led to specific reactivity to CDg4, a glycogen fluorescence sensor, in both human and mouse naïve embryonic stem cells (ESCs). The requirement of constant inhibition of Gsk3β as well as high oxidative phosphorylation (OxPHOS) in naïve compared to primed ESCs was closely associated to high level of intracellular glycogen in naïve ESCs. Both capacity of OxPHOS and stored glycogen, rescued naïve ESCs by transient inhibition of glycolysis, which selectively eliminated primed ESCs. Additionally, naïve ESCs with active OxPHOS were enriched from a mixture with primed ESCs by high reactivity to ATP-Red1, a mitochondrial ATP fluorescence probe. These results indicate the active OxPHOS and high intracellular glycogen as a novel “biomarker” delineating metabolic remodeling during the transition of naïve pluripotency. © 2022 International Metabolic Engineering Society | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | Metabolic Engineering | - |
dc.title | Live isolation of naïve ESCs via distinct glucose metabolism and stored glycogen | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000793674500002 | - |
dc.identifier.doi | 10.1016/j.ymben.2022.03.003 | - |
dc.identifier.bibliographicCitation | Metabolic Engineering, v.72, pp.97 - 106 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85126082575 | - |
dc.citation.endPage | 106 | - |
dc.citation.startPage | 97 | - |
dc.citation.title | Metabolic Engineering | - |
dc.citation.volume | 72 | - |
dc.contributor.affiliatedAuthor | Chun, Kwang-Hoon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Glucose metabolism | - |
dc.subject.keywordAuthor | Glycogen | - |
dc.subject.keywordAuthor | Mitochondrial ATP | - |
dc.subject.keywordAuthor | Naïve pluripotency | - |
dc.subject.keywordAuthor | Primed pluripotency | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | FATTY-ACID SYNTHESIS | - |
dc.subject.keywordPlus | GROUND-STATE | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | LIBRARY | - |
dc.subject.keywordPlus | ESRRB | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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