Establishment of a glycoengineered CHO cell line for enhancing antennary structure and sialylation of CTLA4-Ig
DC Field | Value | Language |
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dc.contributor.author | Lim, Jin-Hyuk | - |
dc.contributor.author | Kim, Jihye | - |
dc.contributor.author | Cha, Hyun-Myoung | - |
dc.contributor.author | Kang, Seung-Hoon | - |
dc.contributor.author | Han, Hye-Jin | - |
dc.contributor.author | Ji, Minkyoo | - |
dc.contributor.author | Cheon, Su-Hwan | - |
dc.contributor.author | Kang, Minju | - |
dc.contributor.author | Kim, Ha Hyung | - |
dc.contributor.author | Kim, Dong-Il | - |
dc.date.accessioned | 2022-06-03T05:40:09Z | - |
dc.date.available | 2022-06-03T05:40:09Z | - |
dc.date.created | 2022-06-03 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84483 | - |
dc.description.abstract | Cytotoxic T-lymphocyte-associated protein 4-Ig (CTLA4-Ig) produced using Chinese hamster ovary (CHO) cell lines is a fusion protein of CTLA4 and the Fc region of antibody. In the present study, we identified and over expressed genes capable of increasing sialic acid levels in CTLA4-Ig to develop cell lines using glycoengineering technology. CTLA4-Ig was produced using CHO cells overexpressing N-acetylglucosaminyltransferase (GnT) and alpha 2,6-sialyltransferase (alpha 2,6-ST). The conditions were wild type (WT), overexpression (GnT-IV, GnT-V, and alpha 2,6-ST), and co-overexpression (GnT-IV and alpha 2,6-ST, and GnT-V and alpha 2,6-ST). GnT-IV and GnT-V were transfected into CHO cells to determine tri-antennary structure formation in CTLA4-Ig. CHOGnT-IV (cells overexpressing GnTIV) showed the highest tri-antennary structures of glycans. Compared to CHOWT, neutral and mono-sialylated glycans decreased (-10.9% and-18.6%, respectively), while bi-and tri-sialylated N-glycans increased (4.1% and 85.7%, respectively) in CHOGnT-IV.ST (cells co-overexpressing GnT-IV and alpha 2,6-ST). The sum of the relative quantities of neutral N-glycans decreased from 32.0% to 28.5%, while that of sialylated N-glycans increased from 68.0% to 71.5% in CHOGnT-IV.ST. These results are the first to demonstrate the co-overexpression of especially GnT-IV and alpha 2,6-ST, which is an effective strategy to increase sialic acid levels and the tri-antennary structure of CTLA4-Ig produced using CHO cell lines. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.title | Establishment of a glycoengineered CHO cell line for enhancing antennary structure and sialylation of CTLA4-Ig | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000795733900001 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2022.110007 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.157 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85125007413 | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 157 | - |
dc.contributor.affiliatedAuthor | Cheon, Su-Hwan | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | CTLA4-Ig | - |
dc.subject.keywordAuthor | CHO cell line | - |
dc.subject.keywordAuthor | Glycoengineering | - |
dc.subject.keywordAuthor | Sialylation | - |
dc.subject.keywordAuthor | Antennary structure | - |
dc.subject.keywordPlus | RECOMBINANT ERYTHROPOIETIN | - |
dc.subject.keywordPlus | FUSION PROTEIN | - |
dc.subject.keywordPlus | N-GLYCANS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | HOST | - |
dc.subject.keywordPlus | GALACTOSYLATION | - |
dc.subject.keywordPlus | GAMMA | - |
dc.subject.keywordPlus | IGG1 | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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