BMP4 signaling plays critical roles in self-renewal of R2i mouse embryonic stem cells
DC Field | Value | Language |
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dc.contributor.author | Taleahmad, Sara | - |
dc.contributor.author | Salari, Ali | - |
dc.contributor.author | Samadian, Azam | - |
dc.contributor.author | Chae, Se Hyun | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Lee, Bonghee | - |
dc.contributor.author | Bayarsaikhan, Delger | - |
dc.contributor.author | Bayarsaikhan, Govigerel | - |
dc.contributor.author | Lee, Jaesuk | - |
dc.contributor.author | Park, Ji Hwan | - |
dc.contributor.author | Hassani, Seyedeh-Nafiseh | - |
dc.contributor.author | Baharvand, Hossein | - |
dc.contributor.author | Salekdeh, Ghasem Hosseini | - |
dc.date.accessioned | 2022-07-19T02:40:24Z | - |
dc.date.available | 2022-07-19T02:40:24Z | - |
dc.date.created | 2022-07-19 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84993 | - |
dc.description.abstract | Mouse embryonic stem cells (mESCs) can be maintained in a pluripotent state under R2i culture conditions that inhibit the TGF-I3 and ERK signaling pathways. BMP4 is another member of the TGF-I3 family that plays a crucial role in maintaining the pluripotency state of mESCs. It has been reported that inhibition of BMP4 caused the death of R2i-grown cells. In this study, we used the loss-of-function approach to investigate the role of BMP4 signaling in mESC self-renewal. Inhibition of this pathway with Noggin and dorsomorphin, two bone morphogenetic protein (BMP) antagonists, elicited a quick death of the R2i-grown cells. We showed that the canonical pathway of BMP4 (BMP/SMAD) was dispensable for self-renewal and maintaining pluripotency of these cells. Transcriptome analysis of the BMPi-treated cells revealed that the p53 signaling and two adhesion (AD) and apoptotic mitochondrial change (MT) pathways could be involved in the cell death of the BMPi-treated cells. According to our results, inhibition of BMP4 signaling caused a decrease in cell adhesion and ECM detachment, which triggered anoikis in the R2i-grown cells. Altogether, these findings demonstrate that endogenous BMP signaling is required for the survival of mESCs under the R2i condition.(c) 2022 Published by Elsevier Inc. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.title | BMP4 signaling plays critical roles in self-renewal of R2i mouse embryonic stem cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000809804300002 | - |
dc.identifier.doi | 10.1016/j.bbrc.2022.05.036 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.617, pp.8 - 15 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85131443458 | - |
dc.citation.endPage | 15 | - |
dc.citation.startPage | 8 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 617 | - |
dc.contributor.affiliatedAuthor | Lee, Bonghee | - |
dc.contributor.affiliatedAuthor | Bayarsaikhan, Delger | - |
dc.contributor.affiliatedAuthor | Bayarsaikhan, Govigerel | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Self-renewal | - |
dc.subject.keywordAuthor | mESCs | - |
dc.subject.keywordAuthor | BMP4 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Cell adhesion | - |
dc.subject.keywordAuthor | RNA-Seq | - |
dc.subject.keywordPlus | ADHESION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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